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CnT-Prime Keratinocyte/Melanocyte Co-Culture Medium

CnT-PR-KM

Frozen medium

500 mL

CHF 260.00

Description

CnT-PR-KM is a fully defined medium specifically formulated to enable balanced co-culture of primary human keratinocytes and melanocytes in a controlled environment. This medium supports the simultaneous growth of both cell types without compromising their respective phenotypes.
Keratinocytes and melanocytes are initially expanded in their respective media—epithelial proliferation media for keratinocytes and CnT-40 for melanocytes. After expansion, cells are seeded at the desired ratio (e.g., 2:1 or 5:1) into standard 2D culture, where both cell types proliferate effectively.
CnT-PR-KM provides an ideal platform for studies involving pigmentation, skin biology, and keratinocyte-melanocyte interactions.

Category

Highlights

1

Balanced keratinocyte–melanocyte co-culture

Specifically engineered for balanced co-culture of keratinocytes and melanocytes

2

Streamlined workflow

Supports proliferation of both cell types after seeding at defined ratios in standard culture

3

Fully defined

Chemically defined, contains no components of animal or human origin, supports 3R principles

4

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

5

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error
free sample

CnT-PR-KM

CnT-NX-E

CnT-40-HC

Tissue type
Skin, Eye
Skin, Bladder, Oral, Other epithelial tissue, Eye
Skin
Cell type
Epithelial Cells, Melanocytes, Keratinocytes
Epithelial Cells, Keratinocytes
Melanocytes
Species
Human, may also work for other species
Human and mouse, may also work for other species
Human, may also work for other species
Workflow Steps
Co-Culture
Isolation, Proliferation
Isolation, Proliferation
Serum Level
Serum-free
Serum-free
Supplementation by user
BPE Level
BPE-free
BPE-free
BPE-free
ACF Status
Free of animal and human components
Free of animal and human components
free of animal and human components
Chemically defined
Yes
Yes
Yes
Clinically upgradable
–
–
–
Volume
500 mL
500 mL
–
Component(s)
–
–
–
Quality Level
Research Grade
Research Grade
Higher Certified
KM_CoCulture_Crop-320x320
[Fig. 1]. CnT-PR-KM delivers balanced co-culture of keratinocytes and melanocytes.

Scientific Literature

Shen Li et al.

(2022)

— Int J Mol Sci

Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma

CnT-40, CnT-PR-KM
Read publication >

Daigo Inoue et al.

(2025)

— Int J Mol Sci

UVB-/Age-Dependent Upregulation of Inflammatory Factor Interleukin-6 Receptor (IL-6R) in Keratinocytes Stimulates Melanocyte Dendricity

CnT-PR-KM
Read publication >

Downloads

CnT-Prime Keratinocyte/Melanocyte Co-Culture Medium Datasheet

Download

CnT-Prime Keratinocyte/Melanocyte Co-Culture Medium SDS

Download

General thawing, passaging, and freezing protocol

Download

Related Products

CnT-PR-KM has been developed for co-culture of keratinocytes and melanocytes in 2D. For 3D co-culture (epidermal models), it is recommended to use CnT-PR-3D. For proliferation, it is recommended to use the respective proliferation media (e.g. CnT-40, CnT-NX-E, CnT-NX-EX).

PR2D-KM

Provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment.

CnT-NX-E

Isolation and rapid expansion of primary epithelial cells from multiple tissue types, iPSC derived cells, and cell lines. Chemically defined, contains no components of animal or human origin.

CnT-40

Selective isolation and rapid expansion of primary epidermal melanocytes.

CnT-NX-EX

Isolation and substantially prolonged expansion of primary epithelial cells derived from multiple tissue types. Chemically defined, contains no components of animal or human origin.

HPEKp

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at early passage.

HEMas

Normal human epidermal melanocytes, isolated from adult donor material, cryo preserved at early passage.

CnT-PR-CC

Co-culture of epithelial and stromal cells in 2D culture.

CnT-PR-3D

Optimal differentiation of primary human epidermal keratinocytes and other epithelial tissues in 3D airlift culture.

PR3D-K-50

Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

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orders@cellntec.com