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CnT-Prime MSC Proliferation Medium, Xeno-Free

CnT-PR-MSC-XF

Frozen medium

500 mL

CHF 334.00

Description

CnT-PR-MSC-XF is a fully xeno-free medium designed for high-efficiency isolation and expansion of mesenchymal stem cells (MSCs) from adipose tissue and bone marrow. It enables robust growth without plate coating and can be upgraded for clinical applications.
Cells cultured in CnT-PR-MSC-XF demonstrate extended longevity and remain strongly multipotent for at least four passages. Adipose-derived MSCs expanded in this medium exhibit the following marker profile: CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, with at least 8% of the population remaining CD34-positive at passage 4. These cells maintain strong multipotency for extended periods and deliver class-leading differentiation efficiency after four passages.
CnT-PR-MSC-XF supports differentiation into adipogenic, osteogenic, and chondrogenic lineages following the addition of the appropriate differentiation supplementation.

Category

Highlights

1

Succesfully isolate and expand MSC

High-efficiency isolation and expansion of adipose and bone-marrow-derived MSC (mesenchymal stromal cells)

2

Xeno-free

Xeno-free, contains no components of animal origin, supports 3R principles

3

Maximize standardization and convenience

Fully supplemented, one bottle ready-to-go. Maximize standardization and convenience. Minimize variability and error

4

Feeder free, no coating required

Stable, feeder-free culture without plate coatings, minimizing batch-to-batch variability and preparation steps

5

Clinically upgradable

Realize a seamless transition from lab to clinical trial and ATMP-development
free sample

CnT-PR-MSC-XF

CnT-PR-MSC

Tissue type
Mesenchyme
Mesenchyme
Cell type
MSCs
MSCs
Species
Human, may also work for other species
Human, may also work for other species
Workflow Steps
Isolation, Proliferation
Isolation, Proliferation
Serum Level
2 % human serum
2 % bovine serum
BPE Level
BPE-free
BPE-free
ACF Status
Xeno-free
Contains animal components
Chemically defined
No
No
Clinically upgradable
–
–
Volume
500 mL
500 mL
Component(s)
–
–
Quality Level
Research Grade
Research Grade
CnT-PR-MSC-XF medium provides faster expansion of bone marrow-derived MSC than widely used alternatives such as αMEM + 10% FCS. The new CnT-Prime MSC-XF medium enables high efficiency isolation and expansion of both adipose and bone marrow derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use!
MSC_XF_400px
[Fig. 2]. BM-MSC (P4) and AD-MSC (P6) growing in CnT-PR-MSC-XF medium.
Adipogenesis_400px
[Fig. 3]. Adipogenesis in BM-MSC (P4). Cells growing in CnT-PR-MSC-XF medium remain strongly multipotent for extended periods. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.
CD34_Cnt-PR-MSC-XF_P4_Web1-320x320
[Fig. 4]. CD34 expression in AD-MSC, CnT-PR-MSC-XF medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept Clinical Research, University of Bern.
MSC_P4_Lipids_CD_SCT-ACF_Web1
[Fig. 5]. Figure: CnT-Prime MSC Media demonstrate high adipogenic differentiation efficiency compared with leading competitors after 4 passages in culture. (A) CnT-PR-MSC-CD, (B) CnT-PR-MSC-XF, (C) Mesencult-ACF.
CFE_XF_P4-320x320
[Fig. 6]. High colony forming efficiency of AD-MSC maintained in CnT-Prime MSC-XF Medium for 4 passages.)

Scientific Literature

Christy Wing Tung Wong et al.

(2022)

— Stem Cell Res Ther

Sourcing of human peripheral blood-derived myeloid angiogenic cells under xeno-free conditions for the treatment of critical limb ischemia

CnT-PR-MSC-XF
Read publication >

Downloads

CnT-Prime MSC Proliferation Medium, Xeno-Free Datasheet

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CnT-Prime MSC Proliferation Medium, Xeno-Free SDS

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General thawing, passaging, and freezing protocol

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Isolation of mesenchymal stem cells from subcutaneous adipose tissue

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