CnT-Dermal Fibroblasts, Adult, Pooled

HDFap

Frozen

Vial containing 0.5 million viable cells

CHF 492.00

Description

HDFap cells are normal human dermal fibroblasts deived from pooled adult donor material and cryopreserved at an early passage. When cultured in CnT‑PR‑F medium, these fibroblasts respond strongly to the proliferative signals provided by the formulation, supporting robust and reliable expansion.
Because this material is of human origin, it must be handled as hazardous. Although the cells have been tested negative for HIV‑1, Hepatitis B, and Hepatitis C, they should be treated as potentially infectious. Appropriate biocontainment procedures, protective equipment, and standard laboratory safety precautions must be used to prevent accidental exposure.

Category Cells

Warning Box

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

Highlights

1

High efficiency

strong growth and extended longevity

2

Specific donor

pooled adult donors

3

High performance

Suitable for complex applications such as 3D differentiation and co-culture

4

Ease of use

Isolated and expanded in CELLnTEC media

5

Performance tested

Cell-culture tested

HDFap

HDFp

HDFs

HDFas

Tissue type

Skin

Skin

Skin

Skin

Cell type

Fibroblasts

Fibroblasts

Fibroblasts

Fibroblasts

Species

Human

Human

Human

Human

Workflow Steps

Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation, Proliferation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Co-Culture, Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models

Serum Level

–

–

–

–

BPE Level

–

–

–

–

ACF Status

–

–

–

–

Chemically defined

–

–

–

–

Clinically upgradable

–

–

–

–

Volume

–

1 mL

1 mL

1 mL

Component(s)

–

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Research Grade

Scientific Literature

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Downloads

CnT-Dermal Fibroblasts, Adult, Pooled Datasheet

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CnT-Dermal Fibroblasts, Adult, Pooled SDS

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General thawing, passaging, and freezing protocol

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Related Products

To ensure optimal results, use recommended culture medium CnT-PR-F.

CnT-PR-F

High efficiency isolation and expansion of fibroblasts, without plate coating.

CnT-PR-ECM

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

CnT-PR-CC

Co-culture of epithelial and stromal cells in 2D culture.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

CnT-PR-FH

Low growth factor medium used for homeostatic maintenance of stromal cells.

HDFas

Normal human dermal fibroblasts, cryo preserved at early passage.

HDFp

Normal human dermal fibroblasts, cryo preserved at early passage.

CnT-Accutase-100

Our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

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