CnT-Dermal Fibroblasts, Juvenile, Pooled

HDFp

Frozen

Vial containing 0.5 million viable cells

CHF 749.00

Description

HDFp cells are normal human dermal fibroblasts derived from pooled juvenile donor material and cryopreserved at an early passage. They deliver strong growth and extended longevity, supported by their juvenile origin and the robust proliferative signals provided by CnT‑PR‑F medium.
Because these cells are of human origin, they must be handled as hazardous. Although tested negative for HIV‑1, Hepatitis B, and Hepatitis C, they should be treated as potentially infectious. Appropriate biocontainment procedures, protective equipment, and standard laboratory safety practices are required to prevent accidental exposure.
The fibroblasts are supplied cryopreserved in fully defined CRYO‑Defined medium to ensure high recovery and viability following thawing.

Category Cells

Warning Box

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

Highlights

1

High efficiency

strong growth and extended longevity

2

Specific donor

pooled juvenile donors

3

High performance

Suitable for complex applications such as 3D differentiation and co-culture

4

Ease of use

Isolated and expanded in CELLnTEC media

5

Performance tested

Cell-culture tested

HDFp

HDFs

HDFas

HDFap

Tissue type

Skin

Skin

Skin

Skin

Cell type

Fibroblasts

Fibroblasts

Fibroblasts

Fibroblasts

Species

Human

Human

Human

Human

Workflow Steps

Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation, Proliferation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Serum Level

–

–

–

–

BPE Level

–

–

–

–

ACF Status

–

–

–

–

Chemically defined

–

–

–

–

Clinically upgradable

–

–

–

–

Volume

1 mL

1 mL

1 mL

–

Component(s)

–

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Research Grade

Scientific Literature

Chiara Griffoni et al.

(2021)

— Sci Rep

In vitro skin culture media influence the viability and inflammatory response of primary macrophages

CnT-PR, CnT-PR-FTAL5, HDFp, HPEKs

Read publication >

Camille Douillet et al.

(2022)

— Biofabrication

From local to global matrix organization by fibroblasts: a 4D laser-assisted bioprinting approach

Read publication >

Rima Nuwayhid et al.

(2024)

— MDPI biomedicines

A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent

HDFp, HPEKp, CnT-PR, CnT-PR-F, CnT-PR-FTAL5

Read publication >

Petra Doerfler et al.

(2024)

— J Investig Dermatol

Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics

Read publication >

Rima Nuwayhid et al.

(2025)

— Front Bioeng Biotechnol

Engineering a 3D wounded skin equivalent to study early inflammatory and regenerative responses in vitro

HPEKp, HDFp, CnT-PR-3D

Read publication >

Downloads

CnT-Dermal Fibroblasts, Juvenile, Pooled Datasheet

Download

CnT-Dermal Fibroblasts, Juvenile, Pooled SDS

Download

General thawing, passaging, and freezing protocol

Download

Related Products

To ensure optimal results, use recommended culture medium CnT-PR-F.

CnT-PR-F

High efficiency isolation and expansion of fibroblasts, without plate coating.

CnT-PR-ECM

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

PR2D-CC

Provides all the components required for establishing balanced co-culture of primary keratinocytes and fibroblasts in a fully defined environment.

PRFT-CELLS-24

Contains everything needed to establish full thickness 3D skin models in your own lab from primary keratinocyte and fibroblast cultures.

CnT-PR-FH

Low growth factor medium used for homeostatic maintenance of stromal cells.

HPEKp

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at early passage.

CnT-Accutase-100

Our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

HDFap

Normal human dermal fibroblasts, cryo preserved at early passage.

HDFs

Normal human dermal fibroblasts, cryo preserved at early passage.

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