CnT-Dermal Fibroblasts, Juvenile, Single Donor

HDFs

Frozen

Vial containing 0.5 million viable cells

CHF 889.00

Description

HDFs are normal human dermal fibroblasts cryopreserved at an early passage. Derived from a single juvenile donor, these cells provide strong growth and extended longevity, particularly when cultured in CnT‑PR‑F medium, which offers potent proliferative support for fibroblast expansion.
Because this material is of human origin, it must be handled as hazardous. Although the cells have been tested negative for HIV‑1, Hepatitis B, and Hepatitis C, they should be treated as potentially infectious. Appropriate biocontainment procedures, protective equipment, and general laboratory safety precautions are required to prevent accidental exposure.
The cells are supplied cryopreserved in fully defined CRYO‑Defined medium to ensure high recovery and viability after thawing.

Category Cells

Warning Box

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

Highlights

1

High efficiency

strong growth and extended longevity

2

Specific donor

Single donor, juvenile

3

High performance

Suitable for complex applications such as 3D differentiation and co-culture

4

Ease of use

Isolated and expanded in CELLnTEC media

5

Performance tested

Cell-culture tested

HDFs

HDFp

HDFas

HDFap

Tissue type

Skin

Skin

Skin

Skin

Cell type

Fibroblasts

Fibroblasts

Fibroblasts

Fibroblasts

Species

Human

Human

Human

Human

Workflow Steps

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Co-Culture, Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models

Co-Culture, Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models

Serum Level

–

–

–

–

BPE Level

–

–

–

–

ACF Status

–

–

–

–

Chemically defined

–

–

–

–

Clinically upgradable

–

–

–

–

Volume

1 mL

1 mL

1 mL

–

Component(s)

–

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Research Grade

Scientific Literature

Wei Long Ng et al.

(2023)

— Bio-Design Manufacturing

Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting

CnT-PR-F, CnT-Accutase-100, HDFs

Read publication >

Kenji Namoto et al.

(2024)

— Cell Stem Cell

NIBR-LTSi is a selective LATS kinase inhibitor activating YAP signaling and expanding tissue stem cells in vitro and in vivo

HPEKas, CnT-PR, HDFs, CnT-PR-FTAL5, CnT-PR-D

Read publication >

Xi Huang et al.

(2023)

— J Intelligent Manufacturing

Predicting the number of printed cells during inkjet-based bioprinting process based on droplet velocity profile using machine learning approaches

HDFs, CnT-PR-F, CnT-Accutase-100

Read publication >

Ruth Jinfen Chai et al.

(2023)

— Int J Bioprinting

Developing a bioink for single-step deposition and maturation of human epidermis

CnT-PR, CnT-PR-F, CnT-PR-3D, HDFs

Read publication >

Downloads

CnT-Dermal Fibroblasts, Juvenile, Single Donor Datasheet

Download

CnT-Dermal Fibroblasts, Juvenile, Single Donor SDS

Download

General thawing, passaging, and freezing protocol

Download

Related Products

To ensure optimal results, use recommended culture medium CnT-PR-F.

CnT-PR-F

High efficiency isolation and expansion of fibroblasts, without plate coating.

CnT-PR-ECM

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

CnT-PR-CC

Co-culture of epithelial and stromal cells in 2D culture.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

CnT-PR-FH

Low growth factor medium used for homeostatic maintenance of stromal cells.

HPEKs

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at at early passage.

HEMns

Normal human epidermal melanocytes, isolated from juvenile donor material, cryo preserved at early passage.

HDFp

Normal human dermal fibroblasts, cryo preserved at early passage.

HDFas

Normal human dermal fibroblasts, cryo preserved at early passage.

Contact Orders Directly