CnT-Epidermal Keratinocyte Progenitors, Adult, Single Donor

HPEKas

Frozen

Vial containing 0.5 million viable cells

CHF 1,384.00

Description

HPEKas are normal human epidermal keratinocyte progenitor cells isolated from adult donor material and cryopreserved at an early passage. They are compatible with several fully defined epithelial media, with CnT‑NX‑E or CnT‑NX‑EX recommended as primary options, and CnT‑PR, CnT‑07, or CnT‑57 available as alternatives.
Because the cells are of human origin, they must be handled as potentially hazardous. Although tested negative for HIV‑1, Hepatitis B, and Hepatitis C, they should be treated as potentially infectious, and appropriate biocontainment, protective equipment, and safety procedures must be followed to prevent accidental exposure.
When cultured in the fully defined, next‑generation CnT‑NX‑E medium, HPEKas benefit from the presence of PCT factors that support retention of proliferative progenitors and delay differentiation. This extends the lifespan of the culture and preserves the cells’ ability to perform complex processes such as 3D differentiation over an extended period.
The cells are supplied cryopreserved in CRYO‑Defined medium to maximize recovery and viability following thawing.

Category Cells

Warning Box

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

Highlights

1

High efficiency

Retention of full proliferation and stratification potential

2

Specific donor

Single donor, adult

3

High performance

Suitable for complex applications such as 3D differentiation

4

Defined cryopreservation

Supplied in fully-defined cryo-preservation medium

5

Ease of use

Isolated and expanded in CELLnTEC media

6

Performance tested

Cell-culture tested

HPEKas

HPEKp

HPEKs

Tissue type

Skin

Skin

Skin

Cell type

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Epithelial Cells, Keratinocytes

Species

Human

Human

Human

Workflow Steps

Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation, Proliferation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation

Serum Level

–

–

–

BPE Level

–

–

–

ACF Status

–

–

–

Chemically defined

–

–

–

Clinically upgradable

–

–

–

Volume

1 mL

1 mL

1 mL

Component(s)

–

–

–

Quality Level

Research Grade

Research Grade

Research Grade

Scientific Literature

Nicolas Mercado et al.

(2019)

— Nat Commun

IRF2 is a master regulator of human keratinocyte stem cell fate

CnT-PR, CnT-Accutase-100, CnT-PR-FTAL5, HPEKs, HPEKas, HDFas

Read publication >

Kenji Namoto et al.

(2024)

— Cell Stem Cell

NIBR-LTSi is a selective LATS kinase inhibitor activating YAP signaling and expanding tissue stem cells in vitro and in vivo

HPEKas, CnT-PR, HDFs, CnT-PR-FTAL5, CnT-PR-D

Read publication >

Downloads

CnT-Epidermal Keratinocyte Progenitors, Adult, Single Donor Datasheet

Download

CnT-Epidermal Keratinocyte Progenitors, Adult, Single Donor SDS

Download

General thawing, passaging, and freezing protocol

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Related Products

To ensure optimal results, use recommended culture media CnT-NX-E or CnT-NX-EX.

CnT-NX-E

Isolation and rapid expansion of primary epithelial cells from multiple tissue types, iPSC derived cells, and cell lines. Chemically defined, contains no components of animal or human origin.

CnT-NX-EX

Isolation and substantially prolonged expansion of primary epithelial cells derived from multiple tissue types. Chemically defined, contains no components of animal or human origin.

HPEKs

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo preserved at at early passage.

PRFT-24

Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.

CnT-PR-D

2D differentiation of epithelial cells in a chemically defined medium.

CnT-CRYO-50

A cryopreservation medium containing all the necessary components to protect cells during freezing and thawing.

CnT-Accutase-100

Our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

CnT-AG2

Specifically designed to age keratinocytes over several passages, without the need for artificial pro-aging treatments.The Vitroage epidermal model maintains normal cell morphology, but generates well documented in vivo signs of ageing including reduced lifespan and disrupted metabolism.

CnT-PR-H

Low growth factor medium for homeostatic maintenance of epithelial cells.

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