CnT-Epidermal Keratinocyte Progenitors, Juvenile, Pooled

HPEKp

Frozen

Vial containing 0.5 million viable cells

CHF 1,391.00

Description

HPEKp cells are normal human epidermal keratinocyte progenitor cells isolated from juvenile donor material and cryopreserved at an early passage. They are suitable for a wide range of epithelial research applications and can be cultured using several fully defined epithelial media. Recommended media include CnT‑NX‑E or CnT‑NX‑EX, with alternative options such as CnT‑PR, CnT‑07, or CnT‑57.
These cells are of human origin and must be handled as potentially hazardous material. Although they have tested negative for HIV‑1, Hepatitis B, and Hepatitis C, appropriate biocontainment procedures, protective equipment, and general precautions must be applied to prevent accidental exposure.
When cultured in the fully defined, next‑generation CnT‑NX‑E medium, HPEKp cells benefit from the presence of PCT factors that support the retention of proliferative progenitors and reduce premature differentiation. This not only extends the overall lifespan of the culture but also preserves the cells’ ability to undertake complex processes such as 3D differentiation over an extended period.
HPEK cells are supplied cryopreserved in CRYO‑Defined medium, which ensures optimal recovery and viability following thawing.

Category

Warning Box

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

Highlights

1

High efficiency

Retention of full proliferation and stratification potential

2

Specific donor

Pooled juvenile donors

3

High performance

Suitable for complex applications such as 3D differentiation

4

Defined cryopreservation

Supplied in fully-defined cryo-preservation medium

5

Ease of use

Isolated and expanded in CELLnTEC media

6

Performance tested

Cell-culture tested

HPEKp

HPEKs

HPEKas

Tissue type
Skin
Skin
Skin
Cell type
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Epithelial Cells, Keratinocytes
Species
Human
Human
Human
Workflow Steps
Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models, Co-Culture
Co-Culture, Homeostasis, Cryopreservation, Proliferation, Differentiation, 3D models
Proliferation, Differentiation, 3D models, Co-Culture, Homeostasis, Cryopreservation
Serum Level
BPE Level
ACF Status
Chemically defined
Clinically upgradable
Volume
1 mL
1 mL
1 mL
Component(s)
Quality Level
Research Grade
Research Grade
Research Grade

Scientific Literature

Cynthia J. Wolf et al.

(2023)

— Birth Defects Res

An improved multicellular human organoid model for the study of chemical effects on palatal fusion

Sara Salman et al.

(2024)

— J Photochemistry Photobiology

Pivotal roles of TRPV1 channel and Nrf2 factor in green light modulation of keratinocyte inflammatory response

Dominik Imfeld et al.

(2024)

— Int J Cosmetic Sci

Boosting of Retinol Activity Using Novel Lecithin: Retinol Acyltransferase Inhibitors

Lisa M. Sevilla et al.

(2024)

— Cell Death Disease

Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes

Omera Bi et al.

(2025)

— Exp Dermatol

Do Melanocytes Have a Role in Controlling Epidermal Bacterial Colonisation and the Skin Microbiome?

Tetsuya Hirabayashi et al.

(2017)

— Nat Commun

PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis

David G. Belair et al.

(2018)

— Toxicological Sci

A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion

David G. Belair et al.

(2017)

— PLoS One

Engineering human cell spheroids to model embryonic tissue fusion in vitro

Downloads

General thawing, passaging, and freezing protocol

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Related Products

To ensure optimal results, use recommended culture media CnT-NX-E or CnT-NX-EX.

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