CnT-Epidermal Keratinocyte Progenitors, Juvenile, Single Donor

HPEKs are normal human epidermal keratinocyte progenitor cells isolated from juvenile donor material and cryopreserved at an early passage. These single‑donor cells can be cultured in several fully defined epithelial media, with CnT‑NX‑E or CnT‑NX‑EX recommended as primary options. Alternative media include CnT‑PR, CnT‑07, or CnT‑57.
Because this material is of human origin, it must be handled as potentially hazardous. Although tested negative for HIV‑1, Hepatitis B, and Hepatitis C, it should be treated as infectious, and appropriate biocontainment procedures, protective equipment, and safety precautions must be used to avoid accidental exposure.
When cultured in fully defined, next‑generation CnT‑NX‑E medium, HPEKs benefit from PCT factors that support the retention of proliferative progenitors and slow the onset of differentiation. This extends the overall lifespan of the culture and preserves the cells’ ability to complete complex processes such as 3D differentiation for a longer period.
The cells are supplied cryopreserved in CRYO‑Defined medium, which ensures high recovery and viability following thawing.