CnT-2D Keratinocyte/Melanocyte Co-Culture Starter Kit, with Cells

The PR2D‑KM 2D Keratinocyte/Melanocyte Co‑Culture Starter Kit provides all components needed to establish a balanced co‑culture of primary keratinocytes and melanocytes in a fully defined environment. The kit includes primary human keratinocyte and melanocyte cells, CnT‑PR and CnT‑40 media for monolayer expansion, and CnT‑PR‑KM co‑culture medium. It is designed to enable the straightforward and reliable establishment of 2D co‑culture models in the laboratory.
Because the primary cells included in the kit are of human origin, they must be handled as hazardous. Although they have been tested negative for HIV‑1, Hepatitis B, and Hepatitis C, they must be treated as potentially infectious, and appropriate biocontainment procedures, protective equipment, and laboratory precautions should be used to prevent accidental exposure.
The keratinocytes and melanocytes are first expanded separately in their respective proliferation media following the recommended general protocol. After expansion, the cells are seeded together at an equivalent ratio in standard 2D culture using CnT‑PR‑KM co‑culture medium, where both cell types proliferate in parallel.
Standard keratinocyte media contain components that inhibit melanocyte growth or induce immediate differentiation, preventing the two cell types from surviving together in conventional 2D culture. In contrast, the CnT‑PR‑KM co‑culture medium included in the kit is specifically tailored to support a stable, balanced co‑culture of primary keratinocytes and melanocytes for up to one week in a fully defined environment.