CnT-3D Epithelial Starter Kit, w/o Cells

The PR3D‑K‑50 3D Keratinocyte Starter Kit provides the media and inserts required to establish three‑dimensional epidermal models with improved differentiation and barrier function in the laboratory. The kit includes CnT‑NX‑E epithelial culture medium for monolayer expansion, CnT‑PR‑3D airlift medium for 3D differentiation, and forty‑eight 0.4 μm pore‑size cell culture inserts designed for air‑lift culture. This version of the kit does not include primary keratinocytes.
To use the kit, early‑passage human keratinocytes cultured in CELLnTEC medium are required. These cells are first expanded in CnT‑NX‑E proliferation medium before being transferred onto the culture inserts. Once established on the inserts, the cells are transitioned to CnT‑PR‑3D barrier medium to support stratification, differentiation, and barrier formation.
When the recommended protocol is followed, the resulting 3D epidermal models deliver up to a 50% improvement in barrier function compared with models generated using traditional culture media. This kit enables reliable, reproducible development of 3D epidermal tissues with enhanced physiological relevance.