CnT-30 is a chemically defined medium for for differentiation of human iPSC and ESC down corneal and epidermal epithelial lineages.

A variety of differentiation protocols have been used succesfully with this product.

It can easily be upgraded for clinical applications. Contact us at scientist@cellntec.com for further details and pricing.

One example of differentiation into corneal epithelium followed by proteomic comparison of iPSC-derived vs primary limbal cells was published in Nature Scientific Reports (Mikhailova et al. 2015).

Other recent papers describing corneal differentiation of hPSCs:

Sorkio et al. 2018, Hongisto et al. 2018.

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Printable datasheet

CnT-Prime iPSC Epithelial Differentiation Medium

CnT-30 is a liquid culture medium kit including both basal medium and supplements. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.

Tissue type
Pack size
CnT-30 is a 500 mL kit consisting of:
  • CnT-BM.1: 500 mL CnT Basal Medium 1 (liquid)
  • CnT-30.S: pack of 2 supplement tube (Supplements A, and B; delivered frozen; to be added to 500 mL basal medium prior to use) 
Product use
Differentiation of human ESC and iPSC down epithelial lineages
Media Type
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
Storage / Shelf life
  • CnT-BM.1 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
  • CnT-30.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
  • The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 6 weeks.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Quality control
Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
  • CnT-BM.1 basal medium is shipped at ambient temperature.
  • CnT-30.S supplements are shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics. For further information about the clinical use of this product, please contact scientist@cellntec.com
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