Contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.
| Catalog | PRFT-24 |
|---|---|
| Content | CnT-PR, CnT-PR-F, CnT-PR-FTAL5, CnT-SP & 24x FT inserts |
Starter kit containing all media necessary for the establishment of 3 dimensional full thickness skin models at the air-liquid interface. The kit includes CnT-PR and CnT-PR-F proliferation media, CnT-PR-FTAL medium for full thickness air-lift culture, CnT-SP spacer plate and 24 cell culture inserts (0.4 um pore size). Kit does not include primary cells.
FT Model established with media included in the PRFT-24 kit.
The PRFT-24 Starter Kit (without cells) contains all the media necessary to establish full thickness 3D skin models from primary keratinocyte and fibroblast cultures in your own lab.
Kit contents include media for 2D proliferation of keratinocytes and fibroblasts, CnT-PR-FTAL5 Medium for full thickness airlift culture, one CnT-SP spacer plate to increase medium volume with standard 12-well plates, plus 24 FT inserts for air-lift culture. For details, please see the datasheet.
Primary keratinocytes and fibroblasts are not included in this kit. These cells can be established in your lab using the CnT-PR and CnT-PR-F media using our recommended protocols, or alternatively they can be purchased together with FT media within the PRFT-CELLS-24 kit, or separately in the CnT-FT-CELLS cell value pack.
The 3D models can be routinely established using our recommended protocol.
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| IRF2 is a master regulator of human keratinocyte stem cell fate | Nicolas Mercado, Gabi Schutzius, Christian Kolter, David Estoppey, Sebastian Bergling, Guglielmo Roma, Caroline Gubser Keller, Florian Nigsch, Adrian Salathe, Remi Terranova, John Reece-Hoyes, John Alford, Carsten Russ, Judith Knehr, Dominic Hoepfner, Alexandra Aebi, Heinz Ruffner, Tanner C. Beck, Sajjeev Jagannathan, Calla M. Olson, Hadley E. Sheppard, Selma Z. Elsarrag, Tewis Bouwmeester, Mathias Frederiksen, Felix Lohmann, Charles Y. Lin, Susan Kirkland | 2019 | Epidermal |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |