- Chemically defined
- High efficiency differentiation
- Extended QC, documentation, support and process control for clinical researchers
Clinically ready, chemically defined, and animal component-free medium for the differentiation of human iPSC and ESC down corneal lineages.
|Content||500 ml basal medium + supplement pack|
CnT-30-HC is a chemically defined and clinically ready liquid culture medium kit for differentiation of human iPSC and ESC down corneal and epidermal epithelial lineages. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.
CnT-30-HC is a chemically defined medium for differentiation of human iPSC and ESC epithelial lineages.
A variety of differentiation protocols have been used succesfully with this product.
One example of differentiation into corneal epithelium followed by proteomic comparison of iPSC-derived vs primary limbal cells was published in Nature Scientific Reports (Mikhailova et al. 2015).
Other recent papers describing corneal differentiation of hPSCs:
Sorkio et al. 2018, Hongisto et al. 2018.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at email@example.com for further details and pricing.
Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
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|Comparative proteomics reveals human pluripotent stem cellderived limbal epithelial stem cells are similar to native ocular surface epithelial cells||Mikhailova, A. et al||2015||Corneal|
|Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells||Heidi Hongisto, Meri Vattulainen, Tanja Ilmarinen, Alexandra Mikhailova, Heli Skottman||2018||Other|
|Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks.||Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H.||2018||Corneal|