- Chemically defined
- High efficiency differentiation
Differentiation of human iPSC and ESC down corneal lineages in a chemically defined, animal component-free medium.
|Content||500 ml basal medium + supplement pack|
CnT-30 is a chemically defined liquid culture medium kit for differentiation of human iPSC and ESC down corneal and epidermal epithelial lineages.
CnT-30 is a chemically defined medium for differentiation of human iPSC and ESC epithelial lineages.
A variety of differentiation protocols have been used succesfully with this product.
One example of differentiation into corneal epithelium followed by proteomic comparison of iPSC-derived vs primary limbal cells was published in Nature Scientific Reports (Mikhailova et al. 2015).
Other recent papers describing corneal differentiation of hPSCs:
Sorkio et al. 2018, Hongisto et al. 2018.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
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|Comparative proteomics reveals human pluripotent stem cellderived limbal epithelial stem cells are similar to native ocular surface epithelial cells||Mikhailova, A. et al||2015||Corneal|
|Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells||Heidi Hongisto, Meri Vattulainen, Tanja Ilmarinen, Alexandra Mikhailova, Heli Skottman||2018||Other|
|Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks.||Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H.||2018||Corneal|