- Chemically defined
- High efficiency differentiation
Differentiation of human iPSC and ESC down corneal lineages in a chemically defined medium.
|Content||500 ml basal medium + supplement pack|
CnT-30 is a chemically defined liquid culture medium kit for differentiation of human iPSC and ESC down corneal and epidermal epithelial lineages.
CnT-30 is a chemically defined medium for differentiation of human iPSC and ESC epithelial lineages.
A variety of differentiation protocols have been used succesfully with this product.
One example of differentiation into corneal epithelium followed by proteomic comparison of iPSC-derived vs primary limbal cells was published in Nature Scientific Reports (Mikhailova et al. 2015).
Other recent papers describing corneal differentiation of hPSCs:
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Like to try a test sample? Click here for more information.
|Comparative proteomics reveals human pluripotent stem cellderived limbal epithelial stem cells are similar to native ocular surface epithelial cells||Mikhailova, A. et al||2015||Corneal|
|Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells with limbal cell characteristics and high wound healing capacity||Meri Vattulainen, Tanja Ilmarinen, Taina Viheriälä, Vilma Jokinen, Heli Skottman||2021||Corneal|
|Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells||Heidi Hongisto, Meri Vattulainen, Tanja Ilmarinen, Alexandra Mikhailova, Heli Skottman||2018||Other|