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CnT-40

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-40 Melanocyte Proliferation Medium

Selective isolation and rapid expansion of primary epidermal melanocytes

Catalog CnT-40
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Very selective medium. Rapid growth & expansion
  • Low serum & does not contain PMA or TPA
  • Fully supplemented, one bottle ready-to-go

Description

CnT-40 is specifically designed for selective isolation and rapid expansion of primary epidermal melanocytes. It can be upgraded for clinical applications.

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Specifications

Tissue type
Melanocytes
Application
Isolation / Expansion
Serum
Low Bovine
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes, Serum needs to be supplemented by the user.
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-40 is designed for high-efficiency isolation and expansion of primary melanocytes. It contains progenitor cell-targeted (PCT) factors to help retain a proliferative phenotype, plus a range of melanocyte-specific factors (including endothelin-1 and endothelin-3) and 1% serum. It does not contain antibiotics / antimycotics and is free of PMA and TPA.

Melanocytes growing in CnT-40 medium display primarily a proliferative bipolar phenotype. The cells retain melanin expression.

We also offer complementary media specifically tailored for melanocyte differentiation (CnT-PR-MD) and whitening studies (CnT-PR-MH). Please see the Melanocyte Page for an overview.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Authors Year Tissue type
Combining bioengineered human skin with bioprinted cartilage for ear reconstruction Dominika Zielinska, Philipp Fisch, Ueli Moehrlen, Sergio Finkielsztein, Thomas Linder, Marcy Zenobi-Wong, Thomas Biedermann, Agnes S. Klar 2023 Epidermal
Efficacy of an agonist of α‐MSH, the palmitoyl tetrapeptide‐20, in hair Pigmentation S. Almeida Scalvino, A. Chapelle, N. Hajem, E. Lati, P. Gasser, J-C. Choulot, L. Michel, M. Hocquaux, E. Loing, J. Attia, J. Wdzieczak-Bakala 2018 Melanocyte
Hypopigmented burn hypertrophic scar contains melanocytes that can be signaled to re-pigment by synthetic alpha-melanocyte stimulating hormone in vitro Bonnie C. Carney, Taryn E. Travis, Lauren T. Moffatt, Laura S. Johnson, Melissa M. McLawhorn, Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, Jeffrey W. Shupp 2021 Melanocyte
Title Year
CELLnTEC Catalog 2023 Download

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