CnT-ENDO

Features

Click to enlarge image. HUVEC growing in CnT-ENDO medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

The CnT-ENDO medium provides class-leading proliferation of primary human endothelial cells over extended periods, all in a FBS-free, clinically upgradable formulation.

Figure: Primary HUVEC cells growing in CnT-ENDO medium at Passage 5 and Passage 9.

Cells demonstrate the correct localisation of VE Cadherin, a critical regulator of endothelial cell behaviour.

Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.

Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.

Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CnT-ENDO medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

Like to try a test sample? Click here for more information.

For an overview of the performance of HUVEC growing in CnT-ENDO medium, please download this A4 Flyer.

CnT-Endothelium Medium

The CnT-Endothelium Medium Medium is a highly refined medium for high efficiency isolation and expansion of primary human endothelial cells. It uses an advanced basal medium supplemented with a range of growth factors, and is FBS-free.

Species

Tested with human cells, may be used with other species as well.

Tissue type

Endothelia

Pack size

500 mL bottle, fully supplemented with growth factors. No further additions required.

Product use

Developed specifically for optimal isolation efficiency and proliferation of human endothelial cells. Recommended seeding density after passaging: 4'000 cells per sq. cm. See the Protocols Page for culture protocols.

Media Type

2D-Prolif

Defined

No

ACF

No

Culture condition

This medium is designed for use in a 5% CO₂ atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com

Note

For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.

Storage / Shelf life

Store frozen below -15 °C. For best before date, see label. To prepare medium for use, thaw in a water bath set to room temperature. Do not use higher temperatures. Swirl frequently, approximately every 20 min, to ensure good mixing of the ingredients and temperature equilibration. Stop at melting of the last bit of ice to prevent warming at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6 weeks when stored at 4 °C in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.

Quality control

Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human cells over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.

Shipping condition

Medium is shipped frozen.

Intended use

For research use only. Not for use in therapy or diagnostics.

Last update

2020-02-20