HIGHLIGHTS:
- Longevity of cells
- Fully supplemented, one bottle ready-to-go
CnT-ENDO Endothelial Proliferation Medium
High-efficiency isolation and expansion of primary human endothelial cells in an FBS-free, clinically upgradable formulation.
| Catalog | CnT-ENDO |
|---|---|
| Content | 500 ml, Frozen medium |
Description
CnT-Endothelium Medium is a highly refined formulation for high-efficiency isolation and expansion of primary human endothelial cells. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free.
Specifications
- Tissue type
- Endothelial cells
- Application
- Isolation / Expansion
- Serum
- Low Human
- BPE-Free
- Yes
- Free of Human & Animal Component
- No
- Chemically defined
- No
- Clinically upgradable
- Yes
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Research Grade
Scientific resources
Figure: Primary HUVEC cells growing in CnT-ENDO medium at Passage 5 and Passage 9.
Primary human endothelial cells growing in CnT-ENDO retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free.
Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.
Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.
Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CnT-ENDO medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
Click to enlarge the image. HUVEC growing in CnT-ENDO medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
For an overview of the performance of HUVEC growing in CnT-ENDO medium, please download this A4 Flyer.
Scientific Literature
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| A microfluidic platform integrating functional vascularized organoids-on-chip | Clément Quintard, Gustav Jonsson, Camille Laporte, Caroline Bissardon, Amandine Pitaval, Nicolas Werschler, Alexandra Leopoldi, Astrid Hagelkrüys, Pierre Blandin, Jean-Luc Achard, Fabrice Navarro,Yves Fouillet, Josef M. Penninger, Xavier Gidrol | 2024 | Other |
| Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model | Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Ga, Gabor Pa, Bence Kiss, Wilbur A. Lam and Verena Schroeder | 2022 | Other |
| Real-time monitoring of oxygen levels within thermoplastic Organ-on-Chip devices | Anubhav Bussooa, Emily Tubbs, Frederic Revol-Cavalier, Ayman Chmayssem, Manuel Alessio, Marie-Line Cosnier, Nicolas Verplanck | 2022 | Other |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |