CELLnTEC / Products / CnT-ISO-50


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-IsoBoost, Isolation Boosting Supplement

A chemically defined supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.

Catalog CnT-ISO-50
Content 50 uL of 1000x concentrate, frozen


  • Chemically defined, contains no components of animal or human origin
  • Significantly increases isolation efficiency


A sterile, 1000 x ready-to-use concentrate which is added to the culture medium for the first 3 days of culture immediately after isolation. Significantly improves viability and attachment of primary human cells.

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Tissue type
Epithelial cells
Free of Human & Animal Component
Chemically defined
Clinically upgradable
50 ul
Quality level
Research Grade

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Scientific resources

CnT-IsoBoost Supplement

Addition of the CnT-IsoBoost supplement to CnT-PR medium for the first 3 days after isolation more than doubles the isolation efficiency.

Isolation is a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.

The IsoBoost supplement (CnT-ISO-50) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress and improving viability and attachment rates.

In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the CnT-ISO-50 supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:

IsoBoost delivers 5x more cells on day 7 post isolation.


CnT-ISO-50-HC delivers 5x more cells on day 7 post isolation.Figure: Primary human keratinocytes (HPEK), day 7 after isolation in CnT-PR plus IsoBoost supplement.

Low cost test samples are available now!

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Scientific Literature

Title Authors Year Tissue type
Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro Sophie Domingues, Annabelle Darle, Yolande Masson, Manoubia Saidani, Emilie Lagoutte, Ana Bejanariu, Julien Coutier, Raif Eren Ayata, Marielle Bouschbacher, Marc Peschanski, Gilles Lemaitre, Christine Baldeschi 2022 Epidermal
Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts Alexis Aguiar, Melissa Galinato, Maite’ Bradley Silva, Bryant Toth, Michael A. McVoy, Laura Hertel 2021 Oral
Introducing a new type of alternative laryngeal mucosa model Tanja Grossmann ,Andrijana Kirsch ,Magdalena Grill,Barbara Steffan,Michael Karbiener,Luka Brcic,Barbara Darnhofer,Ruth Birner-Gruenberger,Markus Gugatschka 2023 Oral
Title Year
CELLnTEC Catalog 2023 Download

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