HIGHLIGHTS:
- Chemically defined, contains no components of animal or human origin
- Significantly increases isolation efficiency
CnT-IsoBoost, Isolation Boosting Supplement
A chemically defined supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.
| Catalog | CnT-ISO-50 |
|---|---|
| Content | 50 uL of 1000x concentrate, frozen |
Description
A sterile, 1000 x ready-to-use concentrate which is added to the culture medium for the first 3 days of culture immediately after isolation. Significantly improves viability and attachment of primary human cells.
Specifications
- Tissue type
- Epithelial cells
- Application
- Isolation
- Serum
- No
- BPE
- No
- Free of Human & Animal Component
- Yes
- Chemically defined
- Yes
- Clinically upgradable
- Yes
- Volume
- 50 ul
- Quality level
- Research Grade
Scientific resources
Addition of the CnT-IsoBoost supplement to CnT-PR medium for the first 3 days after isolation more than doubles the isolation efficiency.
Isolation is a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.
The IsoBoost supplement (CnT-ISO-50) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress and improving viability and attachment rates.
In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the CnT-ISO-50 supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:
CnT-ISO-50-HC delivers 5x more cells on day 7 post isolation.Figure: Primary human keratinocytes (HPEK), day 7 after isolation in CnT-PR plus IsoBoost supplement.
Low cost test samples are available now!
Scientific Literature
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro | Sophie Domingues, Annabelle Darle, Yolande Masson, Manoubia Saidani, Emilie Lagoutte, Ana Bejanariu, Julien Coutier, Raif Eren Ayata, Marielle Bouschbacher, Marc Peschanski, Gilles Lemaitre, Christine Baldeschi | 2022 | Epidermal |
| Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts | Alexis Aguiar, Melissa Galinato, Maite’ Bradley Silva, Bryant Toth, Michael A. McVoy, Laura Hertel | 2021 | Oral |
| Introducing a new type of alternative laryngeal mucosa model | Tanja Grossmann ,Andrijana Kirsch ,Magdalena Grill,Barbara Steffan,Michael Karbiener,Luka Brcic,Barbara Darnhofer,Ruth Birner-Gruenberger,Markus Gugatschka | 2023 | Oral |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |