- Chemically defined, contains no components of animal or human origin
- Significantly increases isolation efficiency
A chemically defined supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.
|Content||50 uL of 1000x concentrate, frozen|
A sterile, 1000 x ready-to-use concentrate which is added to the culture medium for the first 3 days of culture immediately after isolation. Significantly improves viability and attachment of primary human cells.
Isolation is a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.
The IsoBoost supplement (CnT-ISO-50) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress and improving viability and attachment rates.
In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the CnT-ISO-50 supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:
CnT-ISO-50-HC delivers 5x more cells on day 7 post isolation.Figure: Primary human keratinocytes (HPEK), day 7 after isolation in CnT-PR plus IsoBoost supplement.
Low cost test samples are available now!
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