CnT-Prime Co-Culture is a fully defined (serum and BPE-free) medium for the co-culture of primary human keratinocytes and fibroblasts.

Unlike serum-containing media or specialty media designed only for keratinocytes, CnT-PR-CC is specifically designed to encourage balanced growth of the two cell types.

It can easily be upgraded for clinical applications. Contact us at scientist@cellntec.com for further details and pricing.

Primary keratinocytes and fibroblasts are initially expanded in their respective media. When seeded at a 50:50 ratio into standard 2D culture, both cell types proliferate.

At confluency, the cultures display the characteristic keratinocyte progenitor cell colonies (small and tight cobblestone morphology), surounded by fibroblasts (see image below).


Further testing of the co-culture medium with a variety of protocols and culture methods is continuing. Please contact us for latest info, and recommendations for your particular application.

Printable datasheet

CnT-Prime CC, Keratinocyte/Fibroblast Co-Culture Medium

CnT-Prime Co-Culture Medium is specifically formulated to allow balanced co-culture of both epithelial and stromal cells in a fully defined environment.

Developed for primary human epithelial and stromal cells. May be used for other species as well, including mouse.
Tissue type
Pack size
250 mL bottle, fully supplemented with growth factors. No further additions required.
Product use
Developed specifically to maintain balanced co-culture of epithelial and stromal cells. Requires a specific protocol for cell preparation and seeding. Please see the protocol in the resources section of www.cellntec.com for more details.
Media Type
Culture condition
This medium is designed for use in a 5% CO2 atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.
Storage / Shelf life
Store frozen below -15 °C. For best before date, see label. To prepare medium for use, thaw in a water bath set to room temperature. Do not use higher temperatures. Swirl frequently, approximately every 20 min, to ensure good mixing of the ingredients and temperature equilibration. Stop at melting of the last bit of ice to prevent warming at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6 weeks when stored at 4 °C in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
Quality control
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology of primary human epithelial and stromal cells. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Medium is shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
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