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CnT-ABM-10

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Pen-Strep / Amphotericin B Solution, 250 x, Ready-to-Use Single Aliquots

Ready-to-use single aliquots of penicillin G sodium salt, streptomycin sulfate, and amphotericin B (Fungizone) for use as antibiotics/antimycotics during isolation and passage 1.

Catalog CnT-ABM-10
Content 10 x 2.0 mL of frozen concentrate (250 x)

HIGHLIGHTS:

  • Convenient, ready to use aliquots

Description

A 250 x concentrate, sterile solution, ready-to-use single aliquots of penicillin G sodium salt, streptomycin sulfate, and amphotericin B (Fungizone). Working concentration is 100 U / mL penicillin, 100 μg / mL streptomycin and 0.25 μg / mL amphotericin B.

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Specifications

Application
Isolation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
No
Volume
10 x 2 ml
Quality level
Research Grade

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Scientific resources

Antibiotic_Degradation

Gentamycin is resistent to cell culture temperature and pH. In contrast, penicillin and streptomycin are rapidly degraded. Adapted from Schafer et al., J. Appl. Microbiol., 1972.

Traditional antibiotics such as Penicillin / Streptomycin are still widely used in cell culture due to their low cost.

For short periods, they are able to maintain adequate activity. However, beyond 24 hours at the temperatures and pH found in cell culture, their activity decreases rapidly, increasing the chance of selecting resistant bacterial strains.

Where longer periods of activity are required, gentamycin (CnT-GAB10) is recommended.

CnT-ABM10 is provided in convenient, ready to use aliquots.

The use of antibiotics is only recommended during isolation and passage 1. They may disrupt cell bahaviour and are not recommended for use in subsequent passages, or in particular during challenging processes such as differentiation.

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Scientific Literature

Title Authors Year Tissue type
Ameloblastomas Exhibit Stem Cell Potential,Possess Neurotrophic Properties, and EstablishConnections with Trigeminal Neurons Pierfrancesco Pagella, Javier Catón, Christian T. Meisel, Thimios A. Mitsiadis 2020 Dental
Title Year
CELLnTEC Catalog 2020 Download

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