Gentamycin is resistent to cell culture temperature and pH. In contrast, penicillin and streptomycin are rapidly degraded. Adapted from Schafer et al., J. Appl. Microbiol., 1972.
Traditional antibiotics such as Penicillin / Streptomycin are still widely used in cell culture due to their low cost.
For short periods, they are able to maintain adequate activity. However beyond 24 hours at the temperatures and pH found in cell culture, their activity decreases rapidly, increasing the chance of selecting resistent bacterial strains.
Where longer periods of activity are required, gentamycin (CnT-GAB10) is recommended.
CnT-ABM10 is provided in convenient, ready to use aliquots.
The use of antibiotics is only recommended during isolation and passage 1. They may disrupt cell bahaviour and are not recommended for use in subsequent passages, or in particular during challenging processes such as differentiation.
Printable datasheet
Pen / Strep / Amphotericin B Solution, Single Aliquots
250 x concentrate, sterile solution, ready-to-use single aliquots of penicillin G sodium salt, streptomycin sulfate and amphotericin B (Fungizone). Working concentration is 100 U / mL penicillin, 100 μg / mL streptomycin and 0.25 μg / mL amphotericin B.
- Tissue type
- Antibiotics
- Pack size
- 10 x 2.0 mL of frozen concentrate (250 x).
- Defined
- Yes
- ACF
- Yes
- Storage / Shelf life
- Store at -20°C. See label for expiry date.
- Shipping condition
- CnT-ABM10 is shipped frozen.
- Comments
- Working concentration is the generally accepted concentration for this antibiotic / antimycotic combination. Some cells may exhibit various degrees of sensitivity to antibiotics or antimycotics.
- Intended use
- For research use only. Not for use in therapy or diagnostics.
- Last update
- 2017-09-27
