- Gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture
An optimal emzyme for isolation of primary epithelial cells.
|Content||5 x 100 mg (approx) powder aliquots (separate tubes)|
Dispase® is a neutral protease that cleaves fibronectin, collagen IV, and to lesser extent collagen I, and can be used to separate epithelial tissues from underlying mesenchyme.
Dispase II (Neutral Protease) is an optimal enzyme for the isolation of primary epithelial cells.
It provides gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture.
It cleaves fibronectin, collagen IV, and to lesser extent collagen I and can be used to separate epithelial tissues from underlying mesenchyme.
Our dispase product CnT-DNP-10 is provided as 5 x 100 mg (approx) powder aliquots. Powder amounts may vary from lot-to-lot, due to differing activities.
Each 100 mg aliquot is sufficient to make up 40 mL of 1x solution, which is sufficient for 2-4 isolations (depending on the quantity of tissue used). The final 1x concentration after solubilization will be 2.4 U/mL.
With 5 powder aliquots in total, CnT-DNP-10 is thus sufficient for up to 20 isolations.
For recommended isolation protocols, please see the Isolation Protocols page.
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