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CnT-ENDO

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-ENDO Endothelial Proliferation Medium

High-efficiency isolation and expansion of primary human endothelial cells in an FBS-free, Xeno-Free, clinically upgradable formulation.

Catalog CnT-ENDO
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Longevity of cells
  • Fully supplemented, one bottle ready-to-go

Description

CnT-Endothelium Medium is a highly refined formulation for high-efficiency isolation and expansion of primary human endothelial cells. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free.

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Specifications

Tissue type
Endothelial cells
Species
Human, may also work for other species
Application
Isolation / Expansion
Serum
Low Human
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-ENDO_P5_P9
Figure: Primary HUVEC cells growing in CnT-ENDO medium at Passage 5 and Passage 9.

Primary human endothelial cells growing in CnT-ENDO retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free.

Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.

Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.

Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CnT-ENDO medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
Click to enlarge the image. HUVEC growing in CnT-ENDO medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
 
For an overview of the performance of HUVEC growing in CnT-ENDO medium, please download this A4 Flyer.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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