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CnT-GAB-10

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT Gentamycin / Amphotericin B Solution, 500 x, Ready-to-Use Single Aliquots

Convenient and ready to use aliquots of gentamycin/amphotericin to be used as antibiotics/antimycotics that provides broad and stable resistance against contamination for use during isolation and passage 1.

Catalog CnT-GAB-10
Content 10 x 1 mL of frozen concentrate (500 x).

HIGHLIGHTS:

  • Broader effective spectrum
  • Higher thermo-stability at 37°C
  • Longer shelf-life

Description

500 x concentrate, sterile solution, ready-to-use single aliquots of gentamycin sodium salt and amphotericin B (Fungizone). Working concentration is 10 µg / mL gentamycin and 0.25 μg / mL amphotericin B.

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Specifications

Application
Isolation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
No
Volume
10 x 1 mL
Quality level
Research Grade

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Scientific resources

Antibiotic_Degradation

Gentamycin is resistent to cell culture temperature and pH. In contrast, penicillin and streptomycin are rapidly degraded. Adapted from Schafer et al., J. Appl. Microbiol., 1972.

Traditional antibiotics such as Penicillin / Streptomycin are not the best choice for routine cell culture, due to their instability.

In contrast, we recommend the use of Gentamycin, due to the following advantages:

• Broader effective spectrum

• Higher thermo-stability at 37°C (5 days compared to 3 days)

• Longer shelf-life

• Higher acid / alkali resistance (> 90% activity for 2 weeks)

Our Gentamycin/Amphotericin product provides broad and stable resistance against contamination. It is provided in convenient, ready to use aliquots.

The use of antibiotics is only recommended during isolation and passage 1. They may disrupt cell behaviour and are not recommended for use in subsequent passages, or in particular during challenging processes such as differentiation.


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Scientific Literature

Title Authors Year Tissue type
Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts Alexis Aguiar, Melissa Galinato, Maite’ Bradley Silva, Bryant Toth, Michael A. McVoy, Laura Hertel 2021 Oral
SUV39H2 epigenetic silencing controls fate conversion of epidermal stem and progenitor cells Pierre Balmer, William V.J. Hariton, Beyza S. Sayar, Vidhya Jagannathan, Arnaud Galichet, Tosso Leeb, Petra Roosje, Eliane J. Müller 2022 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

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