Gentamycin is resistent to cell culture temperature and pH. In contrast, penicillin and streptomycin are rapidly degraded. Adapted from Schafer et al., J. Appl. Microbiol., 1972.
Traditional antibiotics such as Penicillin / Streptomycin are not the best choice for routine cell culture, due to their instability.
In contrast we recommend the use of Gentamycin, due to the following advantages:
• Broader effective spectrum
• Higher thermo-stability at 37°C (5 days compared to 3 days)
• Longer shelf-life
• Higher acid / alkali resistance (> 90% activity for 2 weeks)
Our Gentamycin/Amphotericin product provides broad and stable resistence against contamination. It is provided in convenient, ready to use aliquots.
The use of antibiotics is only recommended during isolation and passage 1. They may disrupt cell behaviour and are not recommended for use in subsequent passages, or in particular during challenging processes such as differentiation.
Printable datasheet
Gentamycin / Amphotericin B Solution, Single Aliquots
500 x concentrate, sterile solution, ready-to-use single aliquots of gentmycin sodium salt and amphotericin B (Fungizone). Working concentration is 10 µg / mL gentamycin and 0.25 μg / mL amphotericin B.
- Tissue type
- Antibiotics
- Pack size
- 10 x 1 mL of frozen concentrate (500 x).
- Defined
- Yes
- ACF
- Yes
- Storage / Shelf life
- Store at -20°C. See label for expiry date.
- Shipping condition
- CnT-GAB10 is shipped frozen.
- Comments
- Working concentration is the generally accepted and in-house tested concentration for this antibiotic / antimycotic combination. Some cells may exhibit various degrees of sensitivity to antibiotics or antimycotics.
- Intended use
- For research use only. Not for use in therapy or diagnostics.
- Last update
- 2019-09-20
