Addition of the CnT-IsoBoost supplement to CnT-PR medium for the first 3 days after isolation more than doubles the isolation efficiency.
Isolation is a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.
The new IsoBoost supplement (CnT-ISO-50) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress, and improving viability and attachment rates.
In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the CnT-ISO-50 supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:
IsoBoost delivers 5x more cells on day 7 post isolation.
![CnT-ISO-50_HPEK_d7]()
Figure: Primary human keratinocytes (HPEK), day 7 after isolation in CnT-PR plus IsoBoost supplement.
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Printable datasheet
CnT-IsoBoost Supplement
A sterile, 1000 x ready-to-use concentrate which is added to the culture medium for the first 3 days of culture immediately after isolation. Significantly improves viability and attachment of primary human cells.
- Tissue type
- Isolation
- Pack size
- 50 uL of 1000x concentrate. Sufficient to supplement 50 mL of culture medium.
- Defined
- Yes
- ACF
- Yes
- Storage / Shelf life
- Store at -20°C. See label for expiry date. Once thawed, store at 4°C. Use within 4 weeks of thawing.
- Shipping condition
- CnT-ISO-50 is shipped frozen.
- Intended use
- For research use only. Not for use in therapy or diagnostics.
- Last update
- 2017-09-27
