Isolation and rapid expansion of primary epithelial cells from multiple tissue types, iPSC derived cells, and cell lines. Chemically defined, contains no components of animal or human origin, and clinically ready formulation.
| Catalog |
CnT-NX-E-HC |
| Content |
500 ml, Frozen medium |
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HIGHLIGHTS:
- Next generation precision medium in a clinically ready formulation
- Achieve robust isolation and expansion of a broad range epithelial cell types including primary keratinocytes
- Successfully cultivate primary cells, iPSC derived cells, and cell lines
- Delivers excellent morphology and stem cell functionality
- ROCK inhibitor-free, chemically defined, contains no components of animal or human origin
- No feeder cells or plate coating required
- Seamless transition from lab to clinical trial and ATMP-development
- Fully supplemented, one bottle ready-to-go
- Extended QC, documentation, support, and process control for clinical researchers
Description
CnT-NX-E-HC is the next generation successor to CnT-07-HC (and CnT-PR-HC), CELLnTEC’s market leading Higher Certified precision media for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. It features markedly improved expansion rates, robustness and versatility in primary cells, iPSC derived cells and cell lines. CnT-NX-E-HC is a clinically ready formulation that is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.
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Specifications
- Tissue type
- Epithelial cells & Keratinocyte
- Species
- Human, may also work for other species
- Application
- Isolation / Expansion
- Serum-Free
- Yes
- BPE-Free
- Yes
- Free of Human & Animal Component
- Yes
- Chemically defined
- Yes
- Clinically upgradable
- Yes, Clinically Ready
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Higher Certified
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Scientific resources
Primary human adult keratinocytes cultured at passage 4 in CnT-NX-E.
CnT-NX-E and CnT-NX-E-HC have same media formulation and deliver similar results.
CnT-NX-E-HC is Higher Certified version that is chemically defined, low calcium (0.07 mM) medium formulation designed for optimal and rapid isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).It is completely free of animal or human-derived components. CnT-NX-E-HC contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics. This medium is clinically ready formulation provided fully supplemented and frozen.
Primary epithelial cells growing in CnT-NX-E-HC display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.
The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Comparative Analysis of Long-term Cultivation of Adult Keratinocytes: The graph shows adult Keratinocytes cultured in CnT-NX-E medium exhibiting accelerated proliferation rates and achieving a higher number of population doublings in a shorter time frame compared to those grown in our predecessor media, CnT-07 and CnT-PR. CnT-NX-E effectively mitigates the issue of low initial cell counts, allowing for up to a sixfold increase in cell numbers within three passages. CnT-NX-E-HC will deliver similar results like the CnT-NX-E medium.
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Scientific Literature
| Title |
Year |
| CELLnTEC Catalog |
2023 |
Download |
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