CnT-NX-E is a next-generation medium designed for the efficient isolation and expansion of primary epithelial cells from various epithelial tissue types, including keratinocytes from skin, corneal epithelial cells, and oral epithelial cells. It succeeds CELLnTEC’s market leading products CnT-07 and CnT-PR.
CnT-NX-E features advanced proliferation rates and versatility for a wide range of applications for primary cells, iPSC-derived cells, and cell lines. CnT-NX-E medium is chemically defined, and contains no components of animal or human origin. No feeder cells or plate coating are required. The design ensures highest reproducibility and excellent inter-experimental and lot-to-lot consistency. This medium is fully supplemented and comes in a single bottle, ready-to-go.
The medium is ideally suited to support in vitro studies on skin and inflammatory diseases, irritation assays, and cosmetic or drug evaluations, and other 3D-modelling applications. It enables a seamless transition from lab to clinical trial and ATMP-development.
CnT-NX-E supports high cell proliferation rates over several passages. Primary epithelial cells grown in CnT-NX-E exhibit excellent morphology and maintain functionality. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.
The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It can also be procured in an upgraded Higher Certified version for clinical applications.
Like to try a test sample? Click here for more information. Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
