CELLnTEC / Products / CnT-NX-E


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-NX Epithelial Proliferation Medium

Next generation medium for efficient isolation and rapid expansion of epithelial cells from multiple tissue types.

Catalog CnT-NX-E
Content 500 ml, Frozen medium


  • Next generation precision medium for isolation and expansion of epithelial cells
  • Supports primary cells, iPSC derived cells, and cell lines
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go
  • Excellent inter-experimental and lot-to-lot consistency


CnT-NX-E is the next generation successor to CnT-07 and CnT-PR, CELLnTEC’s market leading precision media for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. It features markedly improved expansion rates, robustness and versatility in primary cells, iPSC derived cells and cell lines.

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Tissue type
Epithelial cells
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-NX-E is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal and rapid isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).

It is completely free of animal or human-derived components. CnT-NX-E contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.

This medium is provided fully supplemented and frozen.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-NX-E with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-NX-E display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It can also be procured in an upgraded Higher Certified version for clinical applications.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Year
CELLnTEC Catalog 2023 Download

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