Next generation medium for efficient isolation and rapid expansion of epithelial cells from multiple tissue types. CnT-NX-E effectively mitigates the issue of low initial cell counts, allowing for up to a sixfold increase in cell numbers within three passages.
| Catalog | CnT-NX-E |
|---|---|
| Content | 500 ml, Frozen medium |
CnT-NX-E is the next generation successor to CnT-07 and CnT-PR, CELLnTEC’s market leading precision media for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. It features markedly improved expansion rates, robustness and versatility in primary cells, iPSC derived cells and cell lines.
Primary human adult keratinocytes cultured at passage 4 in the new CnT-NX-E
CnT-NX-E is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal and rapid isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).It is completely free of animal or human-derived components. CnT-NX-E contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.This medium is provided fully supplemented and frozen.
For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-NX-E with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.
Primary epithelial cells growing in CnT-NX-E display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.
The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It can also be procured in an upgraded Higher Certified version for clinical applications.
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Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Comparative Analysis of Long-term Cultivation of Adult Keratinocytes: Adult Keratinocytes cultured in CnT-NX-E medium exhibited accelerated proliferation rates and achieved a higher number of population doublings in a shorter time frame compared to those grown in our predecessor media, CnT-07 and CnT-PR. CnT-NX-E effectively mitigates the issue of low initial cell counts, allowing for up to a sixfold increase in cell numbers within three passages.
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |