CELLnTEC / Products / CnT-PR-3D


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial 3D Airlift Medium

Optimal differentiation of primary human epidermal keratinocytes and other epithelial tissues in 3D airlift culture.

Catalog CnT-PR-3D
Content 500 ml, Frozen medium


  • Superior differentiation and increased barrier function in 3D epidermal keratinocyte models
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go


CnT-PR-3D delivers maximal differentiation and increased barrier function in 3D epidermal keratinocyte models.

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Tissue type
Epithelial cells
Human, may also work for other species
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-Prime 3D Barrier is a chemically defined medium formulation designed for optimal differentiation of primary human epidermal keratinocytes in 3D airlift cultures. It does not contain phenol-red or antibiotics / antimycotics, and is completely free of animal or human-derived components.

CnT-Prime 3D Barrier contains a specifically tailored mix of growth factors and lipids to deliver increased barrier function. It create an environment conducive to full differentiation, and the formation of a strong epidermal barrier (more info).

CnT-PR-3D medium is used to establish 3D epidermal models using primary human keratinocytes expanded in CnT proliferation media (CnT-PR or CnT-07). This can be achieved with 12 days of air-lift culture according to our recommended protocol (see protocol link below).

The 3D Barrier medium is also available as part of our 3D Starter kits, which contain all components necessary for the establishment of 3D epidermal models.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Authors Year Tissue type
A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection Harry Horsley, Dhanuson Dharmasena, James Malone-Lee & Jennifer L. Rohn 2017 Bladder
Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes Lilian Julia Löwenau, Christian Zoschke, Robert Brodwolf, Pierre Volz, Christian Hausmann, Suvara Wattanapitayakul, Alexander Boreham, Ulrike Alexiev, Monika Schäfer-Kortin 2017 Epidermal
QobuR – A new in vitro human corneal epithelial model for preclinical drug screening Manuel Chacón, Natalia Vázquez, Silvia Berisa, Mairobi Persinal, Manuel Sánchez, Begoña Baamonde, Jose F. Alfonso, Luis Fernández-Vega Cueto, Jesús Merayo-Lloves, Álvaro Meana 2019 Corneal

For more publications on CnT-PR-3D from our literature database, see here

Title Year
CELLnTEC Catalog 2020 Download

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