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CnT-PR-CC

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial/Stromal Co-Culture Medium

Co-culture of epithelial and stromal cells in 2D culture.

Catalog CnT-PR-CC
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Specifically designed for balanced co-culture of keratinocytes and fibroblasts
  • Fully supplemented, one bottle ready-to-use

Description

CnT-PR-CC is a fully defined medium for the co-culture of primary human keratinocytes and fibroblasts.

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Specifications

Tissue type
Fibroblasts & Keratinocytes
Application
Co-culture
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

KF_Coculture_1

CnT-PR-CC delivers balanced co-culture of keratinocytes & fibroblasts.

CnT-PR-CC is specifically formulated to allow balanced co-culture of both epithelial and stromal cells in a fully defined environment.Primary keratinocytes and fibroblasts are initially expanded in their respective media. When seeded at a 50:50 ratio into standard 2D culture, both cell types proliferate.

At confluency, the cultures display the characteristic keratinocyte progenitor cell colonies (small and tight cobblestone morphology), surounded by fibroblasts (see image).

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion 2018 Epidermal
Development of an organotypic stem cell model for the study of human embryonic palatal fusion Cynthia J. Wolf, David G. Belair, Carrie M. Becker, Kaberi P. Das, Judith E. Schmid, Barbara D. Abbott 2018 Epidermal
Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks. Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H. 2018 Corneal
Title Year
CELLnTEC Catalog 2020 Download

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