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CnT-PR-D

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial 2D Differentiation Medium

2D differentiation of epithelial cells in a chemically defined medium.

Catalog CnT-PR-D
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Specifically designed for differentiation of epithelial cells
  • Fully supplemented, one bottle ready-to-use

Description

CnT-PR-D is a chemically defined, animal-component free formulation designed for differentiation of epithelial cells from skin, cornea, gingiva, mammary, and bladder cells.

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Specifications

Tissue type
Epithelial cells
Species
Human, may also work for other species
Application
Differentiation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR-D is a chemically defined medium formulation designed for optimal differentiation of primary epithelial cells in 2D culture. It is completely free of animal or human-derived components. This medium is specifically designed to create an environment conducive to full differentiation.

CnT-PR-D contains a specifically tailored mix of growth factors, in which proliferative components like PCT growth factors that retard differentiation are omitted or reduced, and pro-differentiation components are added. It uses an optimized basal medium with additional trace elements, protective antioxidants, and vitamins. It does not contain phenol-red or antibiotics / antimycotics.

It is an evolution and refinement of our previous differentiation media, such as CnT-02, CnT-30 and CnT-32.

CnT-PR-D medium is a low-calcium formulation. Calcium is known to contribute to the differentiation of confluent epithelial cells. It is recommended to add calcium at the desired time-point – please see our corresponding differentiation protocol for our recommendations (protocols link below).

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

 

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Scientific Literature

Title Authors Year Tissue type
Arginine‐ but not alanine‐rich carboxy‐termini trigger nuclear translocation of mutant keratin 10 in ichthyosis with confetti Patricia Renz, Elias Imahorn, Iris Spoerri, Magomet Aushev, Oliver P. March, Hedwig Wariwoda, Sarah Von Arb, Andreas Volz, Peter H. Itin. Julia Reichelt, Bettina Burger 2019 Epidermal
CBP/p300 antagonises EGFR-Ras-Erk signalling and suppresses increased Ras-Erk signalling-induced tumour formation in mice Taeko Ichise, Nobuaki Yoshida, Hirotake Ichise 2019 Epidermal
Gene Editing - Mediated Disruption of Epidermolytic IchthyosiseAssociated KRT10 Alleles Restores Filament Stability in Keratinocytes Oliver P. March, Thomas Lettner, Alfred Klausegger, Michael Ablinger, Thomas Kocher, Stefan Hainzl, Patricia Peking, Nina Lackner, Neil Rajan, Josefina Pinon Hofbauer, Christina Guttmann-Gruber, Anette Bygum, Ulrich Koller, Julia Reichelt 2019 Epidermal

 

Title Year
CELLnTEC Catalog 2020 Download

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