- Chemically defined, contains no components of animal or human origin
- Specifically designed for differentiation of epithelial cells
- Fully supplemented, one bottle ready-to-use
2D differentiation of epithelial cells in a chemically defined medium.
|Content||500 ml, Frozen medium|
CnT-PR-D is a chemically defined, animal-component free formulation designed for differentiation of epithelial cells from skin, cornea, gingiva, mammary, and bladder cells.
CnT-PR-D is a chemically defined medium formulation designed for optimal differentiation of primary epithelial cells in 2D culture. It is completely free of animal or human-derived components. This medium is specifically designed to create an environment conducive to full differentiation.
CnT-PR-D contains a specifically tailored mix of growth factors, in which proliferative components like PCT growth factors that retard differentiation are omitted or reduced, and pro-differentiation components are added. It uses an optimized basal medium with additional trace elements, protective antioxidants, and vitamins. It does not contain phenol-red or antibiotics / antimycotics.
It is an evolution and refinement of our previous differentiation media, such as CnT-02, CnT-30 and CnT-32.
CnT-PR-D medium is a low-calcium formulation. Calcium is known to contribute to the differentiation of confluent epithelial cells. It is recommended to add calcium at the desired time-point – please see our corresponding differentiation protocol for our recommendations (protocols link below).
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
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