CELLnTEC / Products / CnT-PR-ECM


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Fibroblast ECM Medium

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

Catalog CnT-PR-ECM
Content 500 ml, Frozen medium


  • Chemically defined, contains no components of animal or human origin
  • Specifically designed to encourage secretion of ECM proteins
  • Fully supplemented, one bottle ready-to-use


CnT-PR-ECM is a fully defined medium that triggers high ECM production by fibroblasts isolated from a range of tissues.

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Tissue type
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

Isolation Efficiency

Fibroblasts growing in CnT-PR-ECM deliver 5x more collagen than proliferative cells growing in CnT-PR-F.

CnT-PR-ECM is a fully defined medium. It is supplemented with a range of growth factors, and vitamin C. CnT-PR-ECM enables cells to produce in-vivo like quantities of ECM as a result of its altered growth factor mix (which shifts the focus away from strong proliferation), and the presence of vitamin C.When evaluating collagen secretion, it is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant) and to normalize the results for cell number using gDNA.

A complete protocol is available for download.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro Sophie Domingues, Annabelle Darle, Yolande Masson, Manoubia Saidani, Emilie Lagoutte, Ana Bejanariu, Julien Coutier, Raif Eren Ayata, Marielle Bouschbacher, Marc Peschanski, Gilles Lemaitre, Christine Baldeschi 2022 Epidermal
Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents Nikola Kolundzic, Preeti Khurana, Debra Crumrine, Anna Celli, Theodora M. Mauro, Dusko Ilic 2022 Dermal
Human-Induced Pluripotent Stem Cell‒Derived Keratinocytes, a Useful Model to Identify and Explore the Pathological Phenotype of Epidermolysis Bullosa Simplex Julien Coutier, Manon Bonnette, Sabrina Martineau, Aurélie Mercadier, Sophie Domingues, Manoubia Saidani, Margot Jarrige, Hélène Polveche, Annabelle Darle, Nathalie Holic, Smail Hadj-Rabia, Christine Bodemer, Gilles Lemaitre, Cécile Martinat, Christine Baldeschi 2022 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

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