CELLnTEC
CELLnTEC / Products / CnT-PR-ECM

CnT-PR-ECM

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Fibroblast ECM Medium

Encouraging high secretion of extra cellular matrix proteins from stromal cells.

Catalog CnT-PR-ECM
Content 250 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Specifically designed to encourage secretion of ECM proteins
  • Fully supplemented, one bottle ready-to-use

Description

CnT-PR-ECM is a fully defined medium that triggers high ECM production by fibroblasts isolated from a range of tissues.

Back to top

Specifications

Tissue type
Fibroblasts
Species
Human, may also work for other species
Application
Differentiation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
250 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

Back to top

Scientific resources

Isolation Efficiency

Fibroblasts growing in CnT-PR-ECM deliver 5x more collagen than proliferative cells growing in CnT-PR-F.

CnT-PR-ECM is a fully defined medium. It is supplemented with a range of growth factors, and vitamin C. CnT-PR-ECM enables cells to produce in-vivo like quantities of ECM as a result of its altered growth factor mix (which shifts the focus away from strong proliferation), and the presence of vitamin C.

When evaluating collagen secretion, it is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant) and to normalize the results for cell number using gDNA.

A complete protocol is available for download.
 

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Back to top

Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

Back to top