- Chemically defined, contains no components of animal or human origin
- Specifically designed to encourage secretion of ECM proteins
- Fully supplemented, one bottle ready-to-use
Encouraging high secretion of extra cellular matrix proteins from stromal cells.
|Content||500 ml, Frozen medium|
CnT-PR-ECM is a fully defined medium that triggers high ECM production by fibroblasts isolated from a range of tissues.
CnT-PR-ECM is a fully defined medium. It is supplemented with a range of growth factors, and vitamin C. CnT-PR-ECM enables cells to produce in-vivo like quantities of ECM as a result of its altered growth factor mix (which shifts the focus away from strong proliferation), and the presence of vitamin C.When evaluating collagen secretion, it is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant) and to normalize the results for cell number using gDNA.
A complete protocol is available for download.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at email@example.com for further details and pricing.
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Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
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