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CnT-PR-EF

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  • Expansion
  • Differentiation
  • Homeostasis
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  • Staining

CnT-Prime Epithelial Proliferation Medium, EGF/FGF-Free

Differentiation of human iPSC and ESC down corneal lineages in a chemically defined, animal component-free medium.

Catalog CnT-PR-EF
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • High efficiency differentiation

Description

CnT-PR-EF is a chemically defined, animal component-free medium for differentiation of human iPSC and ESC down corneal lineages. This medium contains low growth factors and is free of EGF and EGF to deliver the desired differentiation of iPS cells.

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Specifications

Tissue type
IPSC
Species
Human, may also work for other species
Application
Differentiation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR-EF is a low growth factor culture medium that can be used for the differentiation of iPS cells. It does not contain antibiotics / antimycotics, and is free of EGF and FGF.

For detailed instructions of how CnT-PR-EF can be supplemented to induce iPSC to differentiate into corneal epithelium, please see Nature Protocols (Hayashi et al. 2017).

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Like to try a test sample? Click here for more information.

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Scientific Literature

Title Authors Year Tissue type
Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells Ryuhei Hayashi, Yuki Ishikawa, Ryousuke Katori, Yuzuru Sasamoto, Yuki Taniwaki, Hiroshi Takayanagi, Motokazu Tsujikawa, Kiyotoshi Sekiguchi, Andrew J Quantock, Kohji Nishida 2017 Corneal
Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages Shun Shibata, Ryuhei Hayashi, Toru Okubo, Yuji Kudo, Tomohiko Katayama, Yuki Ishikawa, Junko Toga, Emiko Yagi, Yoichi Honma, Andrew J Quantock, Kiyotoshi Sekiguchi, Kohji Nishida 2018 Other
Title Year
CELLnTEC Catalog 2020 Download

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