- Chemically defined, contains no components of animal or human origin
- High efficiency differentiation
Differentiation of human iPSC and ESC down corneal lineages in a chemically defined, animal component-free medium.
|Content||500 ml, Frozen medium|
CnT-PR-EF is a chemically defined, animal component-free medium for differentiation of human iPSC and ESC down corneal lineages. This medium contains low growth factors and is free of EGF and EGF to deliver the desired differentiation of iPS cells.
CnT-PR-EF is a low growth factor culture medium that can be used for the differentiation of iPS cells. It does not contain antibiotics / antimycotics, and is free of EGF and FGF.
For detailed instructions of how CnT-PR-EF can be supplemented to induce iPSC to differentiate into corneal epithelium, please see Nature Protocols (Hayashi et al. 2017).
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
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|Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells||Ryuhei Hayashi, Yuki Ishikawa, Ryousuke Katori, Yuzuru Sasamoto, Yuki Taniwaki, Hiroshi Takayanagi, Motokazu Tsujikawa, Kiyotoshi Sekiguchi, Andrew J Quantock, Kohji Nishida||2017||Corneal|
|Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities||Shinya Watanabe, Ryuhei Hayashi, Yuzuru Sasamoto, Motokazu Tsujikawa, Bruce R. Ksander, Markus H. Frank, Andrew J. Quantock, Natasha Y. Frank, Kohji Nishida||2021||Other|
|Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages||Shun Shibata, Ryuhei Hayashi, Toru Okubo, Yuji Kudo, Tomohiko Katayama, Yuki Ishikawa, Junko Toga, Emiko Yagi, Yoichi Honma, Andrew J Quantock, Kiyotoshi Sekiguchi, Kohji Nishida||2018||Other|