- Chemically defined, contains no components of animal or human origin
- Low growth factor stimulation increases the sensitivity of assays
- Fully supplemented, one bottle ready-to-use
Low growth factor medium used for homeostatic maintenance of stromal cells.
|Content||250 ml, Frozen medium|
CnT-PR-FH medium is designed for the maintenance of primary human fibroblasts in a homeostatic state with only minimal growth factor stimuli, sufficient to maintain their function for up to a week in culture.
In the absence of the strong growth factor stimulation found in standard proliferation media, fibroblasts growing in CnT-PR-FH show heightened responsiveness to experimental stimuli (for example Vitamin C to stimulate collagen secretion, as shown at left).
Fibroblast responsiveness to vitamin C treatment (100 ug/mL) is 5x higher than in the standard CnT-PR-F proliferation medium. Confluent dermal fibroblasts were switched to the respective media at the confluence and grown for a further 5 days. Total collagen was then lysed in the well (acetic acid), quantified using Sirius red, and normalized to gDNA to correct for any differences in cell number.
It is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant) and to normalize for cell number using gDNA.
A complete protocol is available for download.
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The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details and pricing.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.