- Chemically defined, contains no components of animal or human origin
- Enables the formation of high levels of ECM in the dermis and a fully differentiated epidermis
- No need for collagen gel or scaffolds
Air-lift establishment of full thickness skin models featuring both fibroblasts and keratinocytes.
|Content||500 ml, Frozen medium|
CnT-PR-FTAL5 is a chemically defined medium used during the air-lift phase of full thickness skin model establishment.
CnT-PR-FTAL5 is a chemically defined medium used during air-lift culture period to establish full thickness skin models (3D co-cultures of primary fibroblasts and keratinocytes).
FT models constructed using CnT-Prime Airlift medium can be established simply and reliably without the need for the complication of collagen gels. This is possible as a result of the high levels of ECM secreted by fibroblasts growing in this medium, as is seen in vivo.
The protocol for establishing the models is characterized by 3 key steps:
i) Seed fibroblasts into inserts in CnT-PR-F medium and grow in submerged culture for 9-10 days.
ii) Seed keratinocytes on top of the dermal fibroblast layer and switch to CnT-PR-FTAL medium.
iii) Grow the models for 3 days in submerged culture, then raise to the air-liquid interface for a further 12 days.
A fully detailed protocol for establishing the FT models can be found here.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Keratinocytes and fibroblasts are available separately in the Full Thickness Primary Cell Value Pack (#CnT-FT-CELLS).
For more details about the reusable Spacer Plates (#CnT-SP) that increase medium volume of standard 12-well plates during air-lift culture, please click here.
Free samples of CnT-PR-FTAL5 medium plus 6 inserts are available for selected new customers along with an order for standard CnT-PR expansion medium. Like to try a test sample? Click here for more information.
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|IRF2 is a master regulator of human keratinocyte stem cell fate||Nicolas Mercado, Gabi Schutzius, Christian Kolter, David Estoppey, Sebastian Bergling, Guglielmo Roma, Caroline Gubser Keller, Florian Nigsch, Adrian Salathe, Remi Terranova, John Reece-Hoyes, John Alford, Carsten Russ, Judith Knehr, Dominic Hoepfner, Alexandra Aebi, Heinz Ruffner, Tanner C. Beck, Sajjeev Jagannathan, Calla M. Olson, Hadley E. Sheppard, Selma Z. Elsarrag, Tewis Bouwmeester, Mathias Frederiksen, Felix Lohmann, Charles Y. Lin, Susan Kirkland||2019||Epidermal|