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CnT-PR-H

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial Homeostasis Medium

Low growth factor medium for homeostatic maintenance of epithelial cells.

Catalog CnT-PR-H
Content 250 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Low growth factor stimulation increases the sensitivity of assays
  • Fully supplemented, one bottle ready-to-use

Description

CnT-PR-H medium is designed for the maintenance of primary human keratinocytes in a homeostatic state with only minimal growth factor stimuli, sufficient to maintain their function for up to a week in culture.

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Specifications

Tissue type
Epithelial cells
Species
Human, may also work for other species
Application
Homeostasis
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
250 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

wound-closure_cnt-pr-h

Keratinocytes in a scratch assay show 5x greater responsiveness to the positive control treatment when growing in CnT-PR-H homeostasis medium compared with the standard proliferation medium.

CnT-PR-H is designed for the maintenance of primary keratinocytes in a homeostatic state for up to one week. The low growth-factor environment maximizes responsiveness to experimental stimuli. It does not contain phenol-red or antibiotics / antimycotics.

In the absence of the strong growth factor stimulation found in standard proliferation media, keratinocytes growing in CnT-PR-H showed heightened responsiveness to experimental stimuli. For example, active ingredients stimulate wound closure, as shown in the graph.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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