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CnT-PR-MH

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Melanocyte Homeostasis Medium

Low growth factor medium used for homeostatic maintenance of melanocytes.

Catalog CnT-PR-MH
Content 250 ml, Frozen medium

HIGHLIGHTS:

  • Specifically designed for homeostatic maintenance of melanocytes
  • Low growth factor stimulation increases sensitivity of assays
  • Fully supplemented, one bottle ready-to-use

Description

CnT-PR-MH medium is designed for the maintenance of melanocytes in a homeostatic state with only minimal growth factor stimuli, sufficient to maintain their function for up to a week in culture.

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Specifications

Tissue type
Melanocytes
Species
Human & Mouse
Application
Homeostasis
Serum
Low Bovine
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes, Serum needs to be supplemented by the user.
Volume
250 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

Whitening

Melanocytes growing in CnT-PR-MH are significantly more responsive to treatment with a tyrosinsase inhibitor; demonstrate larger reductions in melanin content in both neonatal and adult cells.

CnT-PR-MH is a fully supplemented, frozen, and ready to use culture medium. It does not contain antibiotics / antimycotics and is free of PMA and TPA.

In the absence of the strong growth factor stimulation found in standard proliferation media, melanocytes growing in CnT-PR-MH showed heightened responsiveness to experimental stimuli (such as tyrosinase inhibitors for example).

To test for whitening of melanocytes, the following procedure is proposed:

i) Grow cells to near confluence in the CnT-40 proliferation medium
ii) Switch cells to CnT-PR-MD medium and allow to differentiate for 5 days
iii) Switch to the Prime MH homeostasis medium one day prior to starting the assay
 
Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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