HIGHLIGHTS:
- Specifically designed for homeostatic maintenance of melanocytes
- Low growth factor stimulation increases sensitivity of assays
- Fully supplemented, one bottle ready-to-use
CnT-Prime Melanocyte Homeostasis Medium
Low growth factor medium used for homeostatic maintenance of melanocytes.
| Catalog | CnT-PR-MH |
|---|---|
| Content | 500 ml, Frozen medium |
Description
CnT-PR-MH medium is designed for the maintenance of melanocytes in a homeostatic state with only minimal growth factor stimuli, sufficient to maintain their function for up to a week in culture.
Specifications
- Tissue type
- Melanocytes
- Application
- Homeostasis
- Serum
- Low Bovine
- BPE
- No
- Free of Human & Animal Component
- No
- Chemically defined
- No
- Clinically upgradable
- Yes, Serum needs to be supplemented by the user.
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Research Grade
Scientific resources
Melanocytes growing in CnT-PR-MH are significantly more responsive to treatment with a tyrosinsase inhibitor; demonstrate larger reductions in melanin content in both neonatal and adult cells.
CnT-PR-MH is a fully supplemented, frozen, and ready to use culture medium. It does not contain antibiotics / antimycotics and is free of PMA and TPA.In the absence of the strong growth factor stimulation found in standard proliferation media, melanocytes growing in CnT-PR-MH showed heightened responsiveness to experimental stimuli (such as tyrosinase inhibitors for example).
To test for whitening of melanocytes, the following procedure is proposed:
i) Grow cells to near confluence in the CnT-40 proliferation medium
ii) Switch cells to CnT-PR-MD medium and allow to differentiate for 5 days
iii) Switch to the Prime MH homeostasis medium one day prior to starting the assay
Like to try a test sample? Click here for more information.
Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Scientific Literature
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |