- Specifically designed for homeostatic maintenance of melanocytes
- Low growth factor stimulation increases sensitivity of assays
- Fully supplemented, one bottle ready-to-use
Low growth factor medium used for homeostatic maintenance of melanocytes.
|Content||500 ml, Frozen medium|
CnT-PR-MH medium is designed for the maintenance of melanocytes in a homeostatic state with only minimal growth factor stimuli, sufficient to maintain their function for up to a week in culture.
CnT-PR-MH is a fully supplemented, frozen, and ready to use culture medium. It does not contain antibiotics / antimycotics and is free of PMA and TPA.In the absence of the strong growth factor stimulation found in standard proliferation media, melanocytes growing in CnT-PR-MH showed heightened responsiveness to experimental stimuli (such as tyrosinase inhibitors for example).
To test for whitening of melanocytes, the following procedure is proposed:
i) Grow cells to near confluence in the CnT-40 proliferation medium
ii) Switch cells to CnT-PR-MD medium and allow to differentiate for 5 days
iii) Switch to the Prime MH homeostasis medium one day prior to starting the assay
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Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.