The new CnT-Prime MSC-XF medium enables high efficiency isolation and expansion of both adipose and bone marrow derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use!
Figure: BM-MSC (P4) and AD-MSC (P6) growing in CnT-PR-MSC-XF medium.
Cells growing in CnT-Prime MSC-XF medium also demonstrate extended longevity, and remain strongly multipotent for at least 4 passages.
Cells can be induced to differentiate down adipogenic, osetogenic or chondrogenic lineages following addition of the corresponding differentiation supplement.
Figure: Adipogenesis in BM-MSC (P4). Cells growing in CnT-PR-MSC-XF medium remain strongly multipotent for extended periods. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.
For an overview of the performance of bone marrow-derived MSC in CnT-PR-XF medium, please download this A4 Flyer.
Adipose-derived MSC expanded in CnT-PR-MSC-XF are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 8% of the cell population remains CD34-positive at passage 4.
Figure: CD34 expression in AD-MSC, CnT-PR-MSC-XF medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept Clinical Research, University of Bern.
AD-MSC expanded in CnT-Prime MSC-XF medium also maintain strong multipotency for extended periods, with class leading differentiation efficiency after 4 passages in culture.
Figure: CnT-Prime MSC Media demonstrate high adipogenic differentiation efficiency compared with leading competitors after 4 passages in culture. (A) CnT-PR-MSC-CD, (B) CnT-PR-MSC-XF, (C) Mesencult-ACF
Figure: High colony forming efficiency of AD-MSC maintained in CnT-Prime MSC-XF Medium for 4 passages.
For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.
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