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CnT-PR-MSC-XF

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime MSC Proliferation Medium, Xeno-Free

High-efficiency xeno-free isolation and expansion of both adipose and bone marrow-derived MSCs, without plate coating.

Catalog CnT-PR-MSC-XF
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Rapid growth & expansion
  • Xeno-free, contains no components of animal origin
  • Fully supplemented, one bottle ready-to-go

Description

The CnT-Prime MSC-XF medium enables high-efficiency isolation and expansion of both adipose and bone marrow-derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use.

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Specifications

Tissue type
MSCs
Species
Human, may also work for other species
Application
Isolation / Expansion
Serum
Low Human
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes, Serum needs to be supplemented by the user.
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR-MSC-XF medium provides faster expansion of bone marrow-derived MSC than widely used alternatives such as αMEM + 10% FCS.

The new CnT-Prime MSC-XF medium enables high efficiency isolation and expansion of both adipose and bone marrow derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use!

MSC_XF Medium
Figure: BM-MSC (P4) and AD-MSC (P6) growing in CnT-PR-MSC-XF medium.

Cells growing in CnT-Prime MSC-XF medium also demonstrate extended longevity and remain strongly multipotent for at least 4 passages.

Cells can be induced to differentiate down adipogenic, osetogenic or chondrogenic lineages following addition of the corresponding differentiation supplement.

Adipogenesis MSC-XF
Figure: Adipogenesis in BM-MSC (P4). Cells growing in CnT-PR-MSC-XF medium remain strongly multipotent for extended periods. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.

For an overview of the performance of bone marrow-derived MSC in CnT-PR-XF medium, please download this A4 Flyer.

Adipose-derived MSC expanded in CnT-PR-MSC-XF are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 8% of the cell population remains CD34-positive at passage 4.
cd34_cnt-pr-msc-xf
Figure: CD34 expression in AD-MSC, CnT-PR-MSC-XF medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept Clinical Research, University of Bern.
 
 
AD-MSC expanded in CnT-Prime MSC-XF medium also maintain strong multipotency for extended periods, with class leading differentiation efficiency after 4 passages in culture.

msc_p4_lipids_cd_sct-acf
 
Figure: CnT-Prime MSC Media demonstrate high adipogenic differentiation efficiency compared with leading competitors after 4 passages in culture. (A) CnT-PR-MSC-CD, (B) CnT-PR-MSC-XF, (C) Mesencult-ACF
 
 

cfe_xf_p4
Figure: High colony forming efficiency of AD-MSC maintained in CnT-Prime MSC-XF Medium for 4 passages.
For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.

Like to try a test sample? Click here for more information.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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