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CELLnTEC / Products / CnT-PR-MSC-XF

CnT-PR-MSC-XF

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime MSC Proliferation Medium, Xeno-Free

High-efficiency xeno-free isolation and expansion of both adipose and bone marrow-derived MSCs, without plate coating.

Catalog CnT-PR-MSC-XF
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Rapid growth & expansion
  • Xeno-free, contains no components of animal origin
  • Fully supplemented, one bottle ready-to-go

Description

The CnT-Prime MSC-XF medium enables high-efficiency isolation and expansion of both adipose and bone marrow-derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use.

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Specifications

Tissue type
MSCs
Application
Isolation / Expansion
Serum
Low Human
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes, Serum needs to be supplemented by the user.
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR-MSC-XF medium provides faster expansion of bone marrow-derived MSC than widely used alternatives such as αMEM + 10% FCS.

The new CnT-Prime MSC-XF medium enables high efficiency isolation and expansion of both adipose and bone marrow derived MSCs, without plate coating, in a fully xeno-free environment that can be upgraded for clinical use!

MSC_XF Medium
Figure: BM-MSC (P4) and AD-MSC (P6) growing in CnT-PR-MSC-XF medium.

Cells growing in CnT-Prime MSC-XF medium also demonstrate extended longevity and remain strongly multipotent for at least 4 passages.

Cells can be induced to differentiate down adipogenic, osetogenic or chondrogenic lineages following addition of the corresponding differentiation supplement.

Adipogenesis MSC-XF
Figure: Adipogenesis in BM-MSC (P4). Cells growing in CnT-PR-MSC-XF medium remain strongly multipotent for extended periods. Images courtesy of Institute of Surgical Technology and Biomechanics, University of Bern.

For an overview of the performance of bone marrow-derived MSC in CnT-PR-XF medium, please download this A4 Flyer.

Adipose-derived MSC expanded in CnT-PR-MSC-XF are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 8% of the cell population remains CD34-positive at passage 4.
cd34_cnt-pr-msc-xf
Figure: CD34 expression in AD-MSC, CnT-PR-MSC-XF medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept Clinical Research, University of Bern.

AD-MSC expanded in CnT-Prime MSC-XF medium also maintain strong multipotency for extended periods, with class leading differentiation efficiency after 4 passages in culture.

msc_p4_lipids_cd_sct-acf

Figure: CnT-Prime MSC Media demonstrate high adipogenic differentiation efficiency compared with leading competitors after 4 passages in culture. (A) CnT-PR-MSC-CD, (B) CnT-PR-MSC-XF, (C) Mesencult-ACF

cfe_xf_p4
Figure: High colony forming efficiency of AD-MSC maintained in CnT-Prime MSC-XF Medium for 4 passages.

For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.Like to try a test sample? Click here for more information.


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Scientific Literature

Title Authors Year Tissue type
Sourcing of human peripheral blood-derived myeloid angiogenic cells under xeno-free conditions for the treatment of critical limb ischemia Christy Wing Tung Wong, Apurva Sawhney, Yalan Wu, Yi Wah Mak, Xiao Yu Tian, Hon Fai Chan, Anna Blocki 2022 Other
Title Year
CELLnTEC Catalog 2023 Download

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