CnT-PR-MSC

Features

Click for enlarged image. CnT-PR-MSC medium provides class-leading adMSC proliferation rates and longevity.

The new CnT-Prime MSC medium offers class-leading culture of both bone marrow and adipose-derived MSCs.

The growth rates of adipose-derived MSC exceeds key competitors (see image at left), all without using plate coatings!

Cells growing in CnT-Prime MSC medium also demonstrate extended longevity, and remain strongly multipotent for at least 4 passages.

It can easily be upgraded for clinical applications. Contact us at scientist@cellntec.com for further details and pricing.

Adipose-derived MSCs grown in CnT-PR-MSC medium are CD45-/CD31-/CD73+/CD90+/CD105+/CD34+/-, and importantly at least 10% of the cell population remains CD34-positive at passage 4.

Figure: CD34 expression in adMSC, CnT-PR-MSC medium, passage 4. Analysis courtesy of Dr Sean Hall, Dept. of Clinical Research, University Hospital Bern.

Cells may at any time be switched to the xeno-free #CnT-PR-MSC-XF medium, or induced to differentiate following addition of the necessary differentiation supplements.

For recommended isolation, expansion, differentiation and CFE protocols, please visit the Protocols Section of this website.

Like to try a test sample? Click here for more information.

CnT-Prime MSC Medium

CnT-Prime MSC Medium is a highly refined medium for high efficiency isolation and expansion of adipose-derived MSC. It uses an advanced basal medium supplemented with a range of growth factors, plus a low level of FCS.

Species

Tested with human cells, may be used with other species as well.

Tissue type

MSC

Pack size

500 mL bottle, fully supplemented with growth factors. No further additions required.

Product use

Developed specifically for optimal isolation efficiency and proliferation of MSCs isolated from bone marrow and adipose tissue. Recommended seeding density of adMSC immediately after isolation: 20'000 cells per sq. cm. Recommended seeding density of adMSC after passaging: 2'000 cells per sq. cm. See the Protocols Page for culture protocols.

Media Type

2D-Prolif

Defined

No

ACF

No

Culture condition

This medium is designed for use in a 5% CO₂ atmosphere. For suggested seeding and culture protocols, please visit the resources section of www.cellntec.com

Note

For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.

Storage / Shelf life

Store frozen below -15 °C. For best before date, see label. To prepare medium for use, thaw in a water bath set to room temperature. Do not use higher temperatures. Swirl frequently, approximately every 20 min, to ensure good mixing of the ingredients and temperature equilibration. Stop at melting of the last bit of ice to prevent warming at the end of the thawing process. Once thawed, medium has a remaining shelf-life of 6 weeks when stored at 4 °C in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.

Quality control

Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human adMSC over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.

Shipping condition

Medium is shipped frozen.

Intended use

For research use only. Not for use in therapy or diagnostics.

Last update

2020-02-20