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CnT-PR

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial Proliferation Medium

Efficient isolation and expansion of epithelial cells from multiple tissue types

Catalog CNT-PR
Content 500 ml, Frozen media

HIGHLIGHTS:

  • Market leading precision medium for isolation and expansion of epithelial cells
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go

Description

CnT-PR is the innovative, market leading precision medium for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue.

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Specifications

Tissue type
Epithelial cells
Species
Human and mouse, may also work for other species
Application
Isolation / Expansion
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).

It is completely free of animal or human-derived components. CnT-Prime contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.

This medium is provided fully supplemented and frozen.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-PR with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-PR display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-PR-HC product page for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

 

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Scientific Literature

Title Authors Year Tissue type
A TALEN-mediated, p63 knock-in application in human iPS cells Kobayashi, Y, Hayashi, R, Quantock, Andrew and Kohji Nishida, K 2017 Corneal
Altered balance of epidermis-related chemokines in epidermolysis bullosa Inkin Ujiie, Yasuyuki Fujita, Chihiro Nakayama, Wakana Matsumura, Shotaro Suzuki, Satoru Shinkuma, Toshifumi Nomura, Riichiro Abe, Hiroshi Shimizu 2017 Epidermal
The secretome of adipose-derived mesenchymal stem cells attenuates epithelial–mesenchymal transition in human corneal epithelium Shun Shibata, Ryuhei Hayashi, Toru Okubo, Yuji Kudo, Koichi Bab, Yoichi Honma, Kohji Nishida 2019 Corneal

 
For more publications on CnT-PR from our literature database, see here
 
 

Title Year
CELLnTEC Catalog 2020 Download

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