CELLnTEC / Products / CnT-PR


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Epithelial Proliferation Medium

Efficient isolation and expansion of epithelial cells from multiple tissue types

Catalog CnT-PR
Content 500 ml, Frozen media


  • Market leading precision medium for isolation and expansion of epithelial cells
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go


CnT-PR is the innovative, market leading precision medium for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue.

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Tissue type
Epithelial cells
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).

It is completely free of animal or human-derived components. CnT-Prime contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.

This medium is provided fully supplemented and frozen.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-PR with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-PR display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-PR-HC product page for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Authors Year Tissue type
A TALEN-mediated, p63 knock-in application in human iPS cells Kobayashi, Y, Hayashi, R, Quantock, Andrew and Kohji Nishida, K 2017 Corneal
Altered balance of epidermis-related chemokines in epidermolysis bullosa Inkin Ujiie, Yasuyuki Fujita, Chihiro Nakayama, Wakana Matsumura, Shotaro Suzuki, Satoru Shinkuma, Toshifumi Nomura, Riichiro Abe, Hiroshi Shimizu 2017 Epidermal
Introducing a new type of alternative laryngeal mucosa model Tanja Grossmann ,Andrijana Kirsch ,Magdalena Grill,Barbara Steffan,Michael Karbiener,Luka Brcic,Barbara Darnhofer,Ruth Birner-Gruenberger,Markus Gugatschka 2023 Oral

For more publications on CnT-PR from our literature database, see here

Title Year
CELLnTEC Catalog 2023 Download

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