- Market leading precision medium for isolation and expansion of epithelial cells
- Chemically defined, contains no components of animal or human origin
- Fully supplemented, one bottle ready-to-go
Efficient isolation and expansion of epithelial cells from multiple tissue types
|Content||500 ml, Frozen media|
CnT-PR is the innovative, market leading precision medium for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue.
CnT-PR is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).
It is completely free of animal or human-derived components. CnT-Prime contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.
This medium is provided fully supplemented and frozen.
For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-PR with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.
Primary epithelial cells growing in CnT-PR display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.
The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-PR-HC product page for further details.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
|A TALEN-mediated, p63 knock-in application in human iPS cells||Kobayashi, Y, Hayashi, R, Quantock, Andrew and Kohji Nishida, K||2017||Corneal|
|Altered balance of epidermis-related chemokines in epidermolysis bullosa||Inkin Ujiie, Yasuyuki Fujita, Chihiro Nakayama, Wakana Matsumura, Shotaro Suzuki, Satoru Shinkuma, Toshifumi Nomura, Riichiro Abe, Hiroshi Shimizu||2017||Epidermal|
|Inflammation and Bone Resorption in a Murine Periodontitis Model||Chiaki Yoshihara-Hirata, Keisuke Yamashiro, Tadashi Yamamoto, Hiroaki Aoyagi, Hidetaka Ideguchi, Mari Kawamura, Risa Suzuki, Mitsuaki Ono, Hidenori Wake, Masahiro Nishibori, Shogo Takashiba||2018||Oral|
For more publications on CnT-PR from our literature database, see here