HIGHLIGHTS:
- Isolation and expansion of epithelial cell types including primary keratinocytes
- Chemically defined, contains no components of animal or human origin
- Fully supplemented, one bottle ready-to-go
Isolation and expansion of epithelial cells from multiple tissue types in a chemically defined environment. Chemically defined and animal-component free.
Catalog | CnT-PR |
---|---|
Content | 500 ml, Frozen medium |
CnT-PR is a fully defined medium for isolation and expansion of a broad range epithelial cell types including primary keratinocytes. CnT-PR medium is chemically defined, and contains no components of animal or human origin. No feeder cells or plate coating are required. The medium is fully supplemented and comes in a single bottle, ready-to-go.
CnT-PR is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal isolation, expansion, and longevity of primary epithelial cells (skin, cornea, oral, mammary).
It is completely free of animal or human-derived components. CnT-Prime contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.
This medium is provided fully supplemented and frozen.
For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-PR with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.
Primary epithelial cells growing in CnT-PR display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.
The medium undergoes a range of QC tests before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-PR-HC product page for further details.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Title | Authors | Year | Tissue type |
---|---|---|---|
A TALEN-mediated, p63 knock-in application in human iPS cells | Kobayashi, Y, Hayashi, R, Quantock, Andrew and Kohji Nishida, K | 2017 | Corneal |
Altered balance of epidermis-related chemokines in epidermolysis bullosa | Inkin Ujiie, Yasuyuki Fujita, Chihiro Nakayama, Wakana Matsumura, Shotaro Suzuki, Satoru Shinkuma, Toshifumi Nomura, Riichiro Abe, Hiroshi Shimizu | 2017 | Epidermal |
Introducing a new type of alternative laryngeal mucosa model | Tanja Grossmann ,Andrijana Kirsch ,Magdalena Grill,Barbara Steffan,Michael Karbiener,Luka Brcic,Barbara Darnhofer,Ruth Birner-Gruenberger,Markus Gugatschka | 2023 | Oral |
For more publications on CnT-PR from our literature database, see here
Title | Year | |
---|---|---|
CELLnTEC Catalog | 2023 | Download |