- High-quality cells
- Pre-equilibrated in CELLnTEC base chemistry
- Guarantee of at least 6 months of growth post-delivery
- Supplied in fully-defined cryopreservation medium to maximize recovery after thawing
Dog epidermal keratinocytes. Long-term culture, cryo-preserved at passage 30 - 40.
|Content||Vial containing 0.5 million viable cells, frozen|
CELLnTEC’s long-term canine epidermal keratinocytes are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.
CPEK long-term canine epidermal keratinocytes are isolated from normal tissue and have not been actively transformed.
CELLnTEC’s Long-Term cells have been widely characterized, provided at approx. passage 30 – 40, and come with a guarantee of at least 6 months of growth post-delivery.
As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.
Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.
|Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1beta, or IFN-gamma in cultured canine keratinocytes||Shibata S, Maeda S, Maeda S, Chimura N, Kondo N, Fukata T.||2010||Epidermal|
|Foxp3+ Regulatory T Cells Associated With CCL17/CCR4 Expression in Carcinomas of Dogs||Shingo Maeda, Maho Nakazawa, Mona Uchida, Ryohei Yoshitake, Takayuki Nakagawa, Ryohei Nishimura, Ryo Miyamoto, Makoto Bonkobara, Tomohiro Yonezawa, Yasuyuki Momoi||2020||Epidermal|
|Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis||Marcella Massimini, Elena Dalle Vedove, Benedetta Bachetti, Francesco Di Pierro, Cataldo Ribecco, Claudio D'Addario, Mariangela Pucci||2021||Epidermal|