Early passage bladder epithelial cells differentiated at the air-liquid interface for 18 days in CnT-PR-D medium. Upper: H&E. Lower: Uroplakin.

3D Differentiation

Differentiation and stratification of epithelial cells in air-lift culture places divergent demands on the culture medium.

The medium must send proliferative signals to retain the basal layer, but must also permit cells to fully differentiate and form an the specialized cells of the upper layers.

The Prime Differentiation medium is specifically designed to deliver this delicate balance. It is able to retain the basal layer, but in parallel allows more complete differentiation.

For complete differentiation, we have observed that keeping the surface of the model moist with some drops of urine has a beneficial effect. Please ask us for more details.