Primary human gingival cells (HGEP) growing in CnT-PR medium. Day 5 after seeding.

Proliferation Media

CnT-Prime medium is carefully formulated to deliver excellent keratinocyte growth and longevity in a fully defined, animal component free environment.

This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.

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Upregulation of differentiation markers (such as desmosomal proteins at cell borders) following switch to CnT-PR-D, and addition of calcium.

Differentiation Media

The differentiation process of epithelial progenitor cells is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation. As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epithelial progenitors, including those isolated from oral tissue.