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HPEKp

CnT Epidermal Keratinocyte Progenitors, Juvenile, Pooled

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo-preserved at early passage.

Catalog HPEKp
Content Vial containing 0.5 million viable cells, frozen

HIGHLIGHTS:

  • Extended lifespan of the cells
  • Ability to complete complex processes such as 3D differentiation
  • Retention of proliferative progenitors and reduced differentiation
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing

Description

HPEKp primary human epidermal keratinocytes (pooled) growing in CnT-PR medium benefit from the presence of PCT factors that aid retention of proliferative progenitors, and reduce differentiation.

HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.

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Specifications

Class
Primary Cells
Tissue type
Skin
Cell type
Epidermal Keratinocyte Progenitors
Donor
Juvenile, pooled donor
Species
Human
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium
CnT-PR / CnT-07 / CnT-57

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Scientific resources

The culturing medium CnT-PR consists of PCT factors that aid retention of proliferative progenitors and reduce differentiation of HPEKp cells. This extends not only the lifespan of the cells but importantly also the period during which the cells retain the ability to complete complex processes such as 3D differentiation.

The HPEK cells are provided cryopreserved in the fully defined CRYO-Defined culture medium to maximize recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale and are also covered by our longevity guarantee. Please see the datasheet for details. Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion 2018 Epidermal
Engineering human cell spheroids to model embryonic tissue fusion in vitro David G. Belair, Cynthia J. Wolf, Carmen Wood, Hongzu Ren, Rachel Grindstaff, William Padgett, Adam Swank, Denise MacMillan, Anna Fisher, Witold Winnik, Barbara D. Abbott 2017 Epidermal
Hypo-osmotic Stress Drives IL-33 Production in Human Keratinocytes—An Epidermal Homeostatic Response Wojciech Pietka, Denis Khnykin, Vibeke Bertelsen, Astrid Haaskjold Lossius, Tor Espen Stav-Noraas, Johanna Hol Fosse, Hilde Kanli Galtung, Guttorm Haraldsen, Olav Sundnes 2019 Epidermal

 
For more publications on HPEK from our literature database, see here
 
 

Title Year
CELLnTEC Catalog 2020 Download

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