House dust mite major allergen Der f 1 enhances proinflammatory cytokine and chemokine gene expression in a cell line of canine epidermal keratinocytes
Authors
Maeda S, Maeda S, Shibata S, Chimura N, Fukata T.
Institution
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
Country
Japan
Year
2009
Journal
Veterinary Immunology and Immunopathology
Abstract
House dust mite (HDM) allergens are the most common allergens involved in the induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD); however, direct stimulation of canine keratinocytes by mite allergens has not been well investigated. In the present study, we investigated the effects of Der f 1, a major allergen of Dermatophagoides farinae, on cytokine and chemokine gene expression in a canine keratinocyte cell line, CPEK. CPEK constitutively expressed mRNA for TNF-alpha, IL-12p35, IL-18, GM-CSF, TGF-beta, IL-8/CXCL8, TARC/CCL17, CTACK/CCL27 and MEC/CCL28. Of all the cytokines and chemokines investigated in CPEK, transcription levels of GM-CSF, IL-8/CXCL8 and TNF-alpha mRNA were significantly enhanced by stimulation with Der f 1. The present results suggest that Der f 1 can directly augment inflammatory cytokine and chemokine production from keratinocytes, and may initiate allergic inflammation independently of Type-I hypersensitivity.
Product use
Cultivation for RT-PCR; mRNA expression of TNF-a, IL-12p35, IL-18, GM-CSF, TGF-b, IL-8/CXCL8, TARC/CCL17, CTACK/CCL27 and MEC/CCL28