Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells
Authors
Sebastian Requena, Olga Ponomarchuk, Marlius Castillo, Jonathan Rebik, Emmanuelle Brochiero, Julian Borejdo, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski, Ryszard Grygorczyk & Rafal Fudala
Institution
University of North Texas Health Science Center
Country
United States
Year
2017
Journal
Nature - Scientific Reports
Abstract
Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases,
including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin
granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to
quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual
granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging
microscopy.