Title
Sphingosine 1-Phosphate (S1P) Induced Interleukin-8 (IL- 8) Release Is Mediated by S1P Receptor 2 and Nuclear Factor kB in BEAS-2B Cells
Authors
Michael J. O’Sullivan, Nobuaki Hirota, James G. Martin
Institution
McGill
Country
Canada
Year
2014
Journal
PLOS
Abstract
The airway epithelium may release pro-inflammatory cytokines and chemokines in the asthmatic airway. Sphingosine 1- phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial cells. S1P is a ligand for 5 G protein-coupled receptors, S1PR1-5. We wished to explore the mechanisms of S1P induced IL-8 secretion with regard to the receptor(s) and downstream signaling events involved. Our results indicate that S1P induced IL-8 release is mediated by S1PR2 and the transcription factor NF-kB. Since the Epidermal Growth Factor Receptor (EGFR) and reactive oxygen species (ROS) have been implicated in IL-8 release in response to activation of other G protein-coupled receptors, we examined their importance in S1P induced IL-8 release and established that they are not involved. This study reveals S1PR2 and NF-kB as potential therapeutic targets in neutrophilic airway diseases such as severe asthma.
Tissue type
Airway
Species
Human
CELLnTEC Previous products
CnT-17, Cell Line

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