Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment
Authors
Laura Frese, Salim E. Darwiche, Brigitte von Rechenberg, Simon P. Hoerstrup, Pietro Giovanoli, Maurizio Calcagni
Institution
University and University Hospital of Zurich; Center for Applied Biotechnology and Molecular Medicine, Zurich
Country
Switzerland
Year
2021
Journal
Cytotherapy
Abstract
Cultured patient-specific keratinocyte sheets have been used clinically since the 1970s for the treatment of large severe burns. However, despite significant developments in recent years, successful and sustainable treatment is still a challenge. Reliable, high-quality grafts with faster availability and a flexible time window for transplantation are required to improve clinical outcomes. The authors demonstrated that temperature is a decisive factor in the production of human keratinocyte sheets.These results show that by using specific temperature ranges, it is possible to accelerate the large-scale production of cultivated keratinocyte sheets while at the same time improving quality. Cultivated keratinocyte sheets are available as early as 18 days post-biopsy and at any time for 7 days thereafter, which increases the flexibility of the process for surgeons and patients alike. These findings will help to provide better clinical outcomes, with an increased take rate in severe burn patients.
Product use
Transport medium for biopsy, keratinocyte culture, keratinocyte sheet production, storage of keratinocyte sheet