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Isolation Protocols
Isolation of primary human dermal fibroblasts
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Isolation of primary human melanocytes
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Isolation of primary human urothelial cells from a bladder biopsy
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Isolation of human corneal epithelial cells
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Isolation of primary human oral epithelial cells
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Isolation of primary human mammary epithelial cells
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Isolation of mesenchymal stem cells from subcutaneous adipose tissue
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Isolation of primary human airway epithelial cells from a section of trachea
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Isolation of primary mouse keratinocytes using CnT-07 medium
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Isolation of primary human keratinocytes
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Proliferation & 2D/3D Differentiation Protocols
2D differentiation of Melanocytes and total melanin assay
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3D differentiation of large airway epithelial cells
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2D differentiation of large airway epithelial cells
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3D Full thickness in vitro skin models at the air-liquid interface
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3D Epidermal in vitro models at the air-liquid interface
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2D keratinocytes differentiation
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Aging of keratinocytes with VitroAge
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General thawing, passaging, and freezing protocol
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Other Cell Culture-related Protocols
Lipid Extraction from 3D epidermal models
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Transfection of epithelial keratinocytes using PEI transfection method
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ECM induction and total collagen measurement
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OCT embedding and cryosectioning of 3D epidermal cultures
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Routine histological processsing, H&E Staining and Immunhistology
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Sample Testing Recommendations
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Thawing Instructions for Frozen Media
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Tissue Type
Name of the tissue type
products used
CnT-PR
CnT-Xp3
CnT-ISO-50
cnt-dnp
cnt-gab-5
cnt-accutase-100
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