Metabolic disruption is a central feature of aging in vivo, that is linked with a variety of other age-related processes. For an overview, please see the review here.
Metabolic disruption is also evident in vitro following aging challenges. This may be easily visualised at the functional level by evaluating cell proliferation.
Alternatively, it may also be evaluated more mechanistically via the integrity of the mitochondrial membrane, the central site of oxidative metabolism in the cell, or using standard metabolism-dependent viability tests such as the MTT test.
Oxidative stress is a well documented contributor to the aging process.
Free radicals generated by oxidative metabolism and exposure to environmental challenges accelerate the aging process. As a result, anti-oxidant active ingredients are able to actively delay the aging process.
The antioxidant capcity of an active ingredient may be evaluated in either keratinocytes or fibroblastsis evaluated following exposure to a peroxide challenge in the presence and absence of the active. After challenge, the residual concentration of oxidative species present in the culture is evaluated using a colorimetric method in 96-well plates.