AIRWAY EPITHELIAL CELL CULTURE

Respiratory disorders, whether acute or chronic, communicable, or noncommunicable, affect millions of people and impose a major global burden on health care system. Respiratory diseases are driven by complex interactions within the host-environment and are presented by structural and functional abnormalities. The lack of reproducible in vitro models that closely reflect in vivo physiology hinders new therapeutic approaches for these diseases.

CELLnTEC OFFERS SPECIALTY MEDIA DESIGNED FOR LARGE AIRWAY EPITHELIAL CELLS.

Our class-leading culture medium has been used for expansion of airway, alveolar and bronchial epithelial cells.

The 3D air–liquid interface (ALI) is a key culture condition to develop and differentiate airway epithelial cells in vitro. 3D ALI models of bronchial cells, upper and lower airway, nasal epithelial cells, and 3D culture of lung epithelial cells are achieved with our chemically defined differentiation medium.

Primary human large airway epithelial
cells proliferation in CnT-PR-A

AIRWAY PROLIFERATION MEDIA

Isolation and expansion of large airway epithelial cells:

CnT-PRIME AIRWAY EPITHELIAL CULTURE MEDIUM (#CnT-PR-A)

Chemically defined, contains no components of animal or human origin
Fully supplemented, one bottle ready-to-go
Evolved successor medium to CnT-17

AIRWAY EPITHELIUM MEDIUM (#CnT-17)

Chemically defined
Liquid culture medium kit including both basal medium and supplements

Both, CnT-PR-A and CnT-17 offer:

Excellent growth and longevity of large airway epithelial cells
PCT factors enhance retention of cells in an undifferentiated phenotype
Co-factors improve growth factor binding and proliferation

ISOLATION PROTOCOLS:

Recommended isolation protocol for large airway epithelial cells

►General Cell Cultivation ►Airway Epithelial Isolation

Human large airway cells differentiation
at the air-liquid interface using CnT-PR-AD

AIRWAY DIFFERENTIATION MEDIUM

Differentiation of large airway epithelial cells. 3D/Barrier/ALI & 2D differentiation:

CnT-PRIME AIRWAY DIFFERENTIATION MEDIUM (#CnT-PR-AD)

Chemically defined, contains no components of animal or human origin
Delivers differentiation and stratification of large airway epithelial cells
Applicable for 2D differentiation or 3D differentiation (Barrier model/ALI culture)
Fully supplemented, one bottle ready-to-go

REAGENTS & COMPLEMENTARY PRODUCTS

2D/3D DIFFERENTIATION PROTOCOLS:

Recommended differentiation protocol to streamline workflow for large airway epithelial cells

►2D Differentiation ►3D Differentiation

NEWSLETTER & SCIENTIFIC LITERATURE

NEWSLETTER EDITIONS DEMONSTRATING USE OF CELLnTEC’s AIRWAY MEDIA:

Newsletter Topics	Published
Establish Lung & Airway Cell Culture and In Vitro Models Successfully	Jan 2023
Successfully transfer serum-dependent cell lines to serum-free defined media	Nov 2022

RECENT PUBLICATIONS CITING CELLnTEC AIRWAY PRODUCTS:

Culture of airway epithelial cells (CnT-PR-A) and lung fibroblasts (CnT-PR-F)	Nkanga et al. 2020
ALI model with human bronchial cells using CnT-PR-AD	Yang et al. 2020
Isolation and culture of Type II Alveolar epithelial cells with CnT-17	Lu et al. 2020
Culture and assays with immortalized lung epithelial cells with CnT-17	Bogucka et al. 2020
Isolation and culture of Human bronchial epithelial cells with CnT-PR-A	Roth et al. 2021
3D sphere formation with CnT-PR-A	Deng et al. 2022

3D ORGANOIDS DEVELOPED WITH CELLnTEC’s AIRWAY MEDIA FOR EVALUATING THE POTENTIAL OF CELL THERAPY TO TREAT CHRONIC RESPIRATORY DISEASES SUCH AS ASTHMA, COPD, PULMONARY AND CYSTIC FIBROSIS:

3D Organotypic Model	Article Link
3D cultured human lung epithelial cells differentiated to form bronchiole-like and alveolus-like organoids	Tanaka et al. 2018
3D ALI murine lung scaffolds to induce embryonic stem cells to differentiate into various types of lung cells	Kawai et al. 2018

CELLnTEC’s AIRWAY MEDIA DEPLOYED FOR 2D/3D CULTURING OF AIRWAY CELLS FROM DIFFERENT SPECIES FOR RESPIRATORY VIRAL RESEARCH:

Airway Cell Culture System	Respiratory Virus	Article Link
Primary human airway epithelial cells	SARS-CoV-2 virus	Ding et al. 2022
3D ALI models of human upper and lower airway	Pandemic influenza A, H1N1pdm	Xia et al. 2020
Primary human bronchial epithelial cells	Human rhinovirus	Roth et al. 2017
Triple co-culture of human respiratory tract	Influenza virosomes or liposomes	Blom et al. 2016
3D airway culture from Ferret trachea	Human Influenza A or B viruses	Elderfield et al. 2015
3D ALI culture of human nasal epithelial cells	Murine-adapted influenza virus	Kumar et al. 2011
Avian tracheal epithelial cell culture system	Infectious bronchitis virus	Shen et al. 2010
Monolayers of murine primary airway epithelial cells	Human rhinovirus	Brockman-Schneider et al. 2008

Xia et al. demonstrated that 3D ALI cultures of the human lower airway track epithelial cells expressed a higher level of viral receptor ACE2 mRNA as compared to the upper airway cells, suggesting that these 3D models are well-suited for studying SARS-CoV-2 coronavirus.

CUSTOMER FEEDBACK

“We have tested several commercially available media for selection and growth of human airway epithelial cells and in our hands the CnT-Prime Airway medium is the best in class”

– [Dr Sean Hall, Department of Clinical Research, University Hospital Bern]

TECHNICAL SUPPORT:

Contact scientists in our R&D team for support in accelerating scientific discovery

ORDERING:

Order Airway products directly or via our distributors network

Epithelial Airway Research